Distribution of G/sub o. cap alpha. / mRNA and protein in bovine tissues

Price, S R; Tsai, S C; Adamik, R; Angus, C W; Van Meurs, K P; Czarnecki, S; Bruckwick, E C; Moss, J; Vaughan, M

Title: Distribution of G/sub o. cap alpha. / mRNA and protein in bovine tissues

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Abstract

G/sub o..cap alpha../ is a 39 kDa guanyl nucleotide-binding protein similar in structure and function to G/sub s..cap alpha../ and G/sub i..cap alpha../ in the adenylate cyclase complex and transducin (G/sub t..cap alpha../) in the retinal photon receptor system. A bovine retinal cDNA clone, lambdaG09, that encodes the complete amino acid sequence of G/sub o..cap alpha../ has been isolated. Nick-translated lambdaG09 cDNA and a 5' end-labeled oligonucleotide probe complementary to a 24 base sequence unique to G/sub o..cap alpha../ were used as probes for Northern analysis of poly(A)/sup +/ RNA from bovine tissues. A major 4.0 kb mRNA was detected in brain and retina and in lesser amounts in heart. Several smaller mRNAs also hybridized with both probes in these tissues and in liver and lung. G/sub o..cap alpha../ protein was identified using rabbit polyclonal antibodies directed against purified bovine G/sub o..cap alpha../ and pertussis toxin-catalyzed (/sup 32/P)ADP-ribosylation. Soluble and membrane proteins were incubated with toxin and (/sup 32/P)NAD and then separated by gel electrophoresis before transfer to nitrocellulose for immunoreaction and subsequent autoradiography. A radiolabeled and immunoreactive 39 kDa membrane protein was found principally in retina and brain, and to a lesser extent, in heart. Thus, in the tissuesmore »« less

Authors:: Price, S R; Tsai, S C; Adamik, R; Angus, C W; Van Meurs, K P; Czarnecki, S; Bruckwick, E C; Moss, J; Vaughan, M

Publication Date:: Fri May 01 00:00:00 EDT 1987

Research Org.:: NIH, Bethesda, MD

OSTI Identifier:: 6126193

Report Number(s):: CONF-870644-
Journal ID: CODEN: FEPRA; TRN: 87-034034

Resource Type:: Conference

Journal Name:: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

Additional Journal Information:: Journal Volume: 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; MESSENGER-RNA; HYBRIDIZATION; TISSUE DISTRIBUTION; PROTEINS; ADP; ANTIBODIES; AUTORADIOGRAPHY; CATTLE; COMPARATIVE EVALUATIONS; ELECTROPHORESIS; NAD; PHOSPHORUS 32; TOXINS; ANIMALS; ANTIGENS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; COENZYMES; DAYS LIVING RADIOISOTOPES; DISTRIBUTION; DOMESTIC ANIMALS; ISOTOPES; LIGHT NUCLEI; MAMMALS; MATERIALS; NUCLEI; NUCLEIC ACIDS; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; RADIOISOTOPES; RNA; RUMINANTS; TOXIC MATERIALS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

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                    Price, S R, Tsai, S C, Adamik, R, Angus, C W, Van Meurs, K P, Czarnecki, S, Bruckwick, E C, Moss, J, and Vaughan, M. Distribution of G/sub o. cap alpha. / mRNA and protein in bovine tissues.  United States: N. p., 1987. 
        Web.

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                    Price, S R, Tsai, S C, Adamik, R, Angus, C W, Van Meurs, K P, Czarnecki, S, Bruckwick, E C, Moss, J, & Vaughan, M. Distribution of G/sub o. cap alpha. / mRNA and protein in bovine tissues.  United States.

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                    Price, S R, Tsai, S C, Adamik, R, Angus, C W, Van Meurs, K P, Czarnecki, S, Bruckwick, E C, Moss, J, and Vaughan, M. 1987.  
        "Distribution of G/sub o. cap alpha. / mRNA and protein in bovine tissues".  United States.

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@article{osti_6126193,

  title        = {Distribution of G/sub o. cap alpha. / mRNA and protein in bovine tissues},

  author       = {Price, S R and Tsai, S C and Adamik, R and Angus, C W and Van Meurs, K P and Czarnecki, S and Bruckwick, E C and Moss, J and Vaughan, M},

  abstractNote = {G/sub o..cap alpha../ is a 39 kDa guanyl nucleotide-binding protein similar in structure and function to G/sub s..cap alpha../ and G/sub i..cap alpha../ in the adenylate cyclase complex and transducin (G/sub t..cap alpha../) in the retinal photon receptor system. A bovine retinal cDNA clone, lambdaG09, that encodes the complete amino acid sequence of G/sub o..cap alpha../ has been isolated. Nick-translated lambdaG09 cDNA and a 5' end-labeled oligonucleotide probe complementary to a 24 base sequence unique to G/sub o..cap alpha../ were used as probes for Northern analysis of poly(A)/sup +/ RNA from bovine tissues. A major 4.0 kb mRNA was detected in brain and retina and in lesser amounts in heart. Several smaller mRNAs also hybridized with both probes in these tissues and in liver and lung. G/sub o..cap alpha../ protein was identified using rabbit polyclonal antibodies directed against purified bovine G/sub o..cap alpha../ and pertussis toxin-catalyzed (/sup 32/P)ADP-ribosylation. Soluble and membrane proteins were incubated with toxin and (/sup 32/P)NAD and then separated by gel electrophoresis before transfer to nitrocellulose for immunoreaction and subsequent autoradiography. A radiolabeled and immunoreactive 39 kDa membrane protein was found principally in retina and brain, and to a lesser extent, in heart. Thus, in the tissues examined, distribution of the 4.0 kb mRNA parallels that of the immunoreactive G/sub o..cap alpha../ with relatively small amounts in heart and larger amounts in brain and retina.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/6126193},
  journal      = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number       = ,

  volume       = 46:6,

  place        = {United States},

  year         = {Fri May 01 00:00:00 EDT 1987},

  month        = {Fri May 01 00:00:00 EDT 1987}

}

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