Liquid scintillation counting for /sup 14/C uptake of single algal cells isolated from natural samples

Journal Article · Wed Jul 01 00:00:00 EDT 1981 · Limnol. Oceanogr.; (United States)

DOI:https://doi.org/10.4319/lo.1981.26.4.0780· OSTI ID:6123545

Rivkin, R B; Seliger, H H

Short term rates of /sup 14/C uptake for single cells and small numbers of isolated algal cells of five phytoplankton species from natural populations were measured by liquid scintillation counting. Regression analysis of uptake rates per cell for cells isolated from unialgal cultures of seven species of dinoflagellates, ranging in volume from ca. 10/sup 3/ to 10/sup 7/ ..mu..m/sup 3/, gave results identical to uptake rates per cell measured by conventional /sup 14/C techniques. Relative standard errors or regression coefficients ranged between 3 and 10%, indicating that for any species there was little variation in photosynthesis per cell.

• Research Organization: Johns Hopkins Univ., Baltimore, MD
• DOE Contract Number: EY-76-S-20-3278
• OSTI ID: 6123545
• Journal Information: Limnol. Oceanogr.; (United States), Vol. 26:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PHOTOSYNTHESIS
MEASURING METHODS
PHYTOPLANKTON
GROWTH
AQUATIC ECOSYSTEMS
CARBON 14 COMPOUNDS
ECOLOGY
LIQUID SCINTILLATION DETECTORS
SCINTILLATION COUNTING
TRACER TECHNIQUES
UPTAKE
AQUATIC ORGANISMS
CHEMICAL REACTIONS
COUNTING TECHNIQUES
ECOSYSTEMS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MEASURING INSTRUMENTS
PHOTOCHEMICAL REACTIONS
PLANKTON
RADIATION DETECTORS
SCINTILLATION COUNTERS
SYNTHESIS
550101* - Behavioral Biology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques