Quantitation of cytochrome P-450 form 3a mRNA in tissues from untreated and ethanol-, acetone-, and imidazole-treated rabbits
This laboratory recently cloned and sequenced several overlapping cDNAs encoding rabbit liver cytochrome P-450 form 3a (P-450/sub alc/). In the present study the relative levels of the mRNA encoding this P-450 were determined in various rabbit tissues by slot-blot hybridization and scanning densitometry, with a 3'-nontranslated portion of the form 3a cDNA used as a probe. Male New Zealand White rabbits were either untreated or administered ethanol, acetone, or imidazole, and total cellular RNA was prepared by homogenization in guanidinium isothiocyanate and sedimentation through CsCl. With untreated animals, the P-450 3a probe hybridized most strongly to RNA isolated from liver, with considerably less hybridization to RNA from kidney, testis, lung, adrenal, and intestine. Treatment with imidazole or acetone resulted in a 1.6-fold and 1.9-fold increase, respectively, in the level of hybridizable RNA from liver, but did not affect the levels of P-450 3a RNA from the other tissues. Surprisingly, ethanol treatment resulted in a 50% decrease in the levels of 3a RNA from liver and kidney, while other tissue 3a RNA levels were unchanged. This effect is in marked contrast to the 3-fold and 5-fold increase in 3a protein in liver and kidney, respectively, after ethanol treatment.
- Research Organization:
- Univ. of Michigan, Ann Arbor
- OSTI ID:
- 6119978
- Report Number(s):
- CONF-870644-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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MESSENGER-RNA
HYBRIDIZATION
TISSUE DISTRIBUTION
ACETONE
ADRENAL GLANDS
ETHANOL
IMIDAZOLES
INTESTINES
KIDNEYS
LIVER
LUNGS
PROTEINS
RABBITS
ALCOHOLS
ANIMALS
AZOLES
BODY
DIGESTIVE SYSTEM
DISTRIBUTION
ENDOCRINE GLANDS
GASTROINTESTINAL TRACT
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
KETONES
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RESPIRATORY SYSTEM
RNA
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology