Phylogenetic relationships among subsurface microorganisms

Technical Report · Tue Jan 01 00:00:00 EST 1991

DOI:https://doi.org/10.2172/6106595· OSTI ID:6106595

Nierzwicki-Bauer, S A

This project involves the development of group specific 16S ribosomal RNA-targeted oligonucleotide hybridization probes for the rapid detection of specific types of subsurface organisms (e.g., groups of microbes that share certain physiological traits). Major accomplishments for the period of 6/91 to 12/1/91 are described. Nine new probes have been synthesized on the basis of published 16S rRNA sequence data from the Ribosomal Database Project. We have initiated rapid screening of many of the subsurface microbial isolates obtained from the P24 borehole at the Savannah River Site. To date, we have screened approximately 50% of the isolates from P24. We have optimized our {und in situ} hybridization technique, and have developed a cell blot hybridization technique to screen 96 samples on a single blot. This is much faster than reading 96 individual slides. Preliminary experiments have been carried out which indicate specific nutrients can be used to amplify rRNA only in those organisms capable of metabolizing those nutrients. 1 tab., 2 figs.

• Research Organization: Rensselaer Polytechnic Inst., Troy, NY (United States)
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-90ER60989
• OSTI ID: 6106595
• Report Number(s): DOE/ER/60989-2; ON: DE92004421
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
54 ENVIRONMENTAL SCIENCES
BACTERIA
TAXONOMY
RIBOSOMAL RNA
HYBRIDIZATION
SAVANNAH RIVER PLANT
SUBSURFACE ENVIRONMENTS
BIOGEOCHEMISTRY
BOREHOLES
DNA SEQUENCING
GENETICS
MOLECULAR BIOLOGY
NUTRIENTS
OLIGONUCLEOTIDES
PROGRESS REPORT
SPECIES DIVERSITY
BIOLOGY
CAVITIES
CHEMISTRY
DOCUMENT TYPES
GEOCHEMISTRY
MICROORGANISMS
NATIONAL ORGANIZATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RNA
STRUCTURAL CHEMICAL ANALYSIS
US AEC
US DOE
US ERDA
US ORGANIZATIONS
550700* - Microbiology
550400 - Genetics
550200 - Biochemistry
540250 - Environment
Terrestrial- Site Resource & Use Studies- (1990-)