Differential identification of Candida species and other yeasts by analysis of (/sup 35/S)methionine-labeled polypeptide profiles

Journal Article · Thu Dec 01 00:00:00 EST 1988 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(88)90582-9· OSTI ID:6101683

Shen, H D; Choo, K B; Tsai, W C; Jen, T M; Yeh, J Y; Han, S H

This paper describes a scheme for differential identification of Candida species and other yeasts based on autoradiographic analysis of protein profiles of (/sup 35/S)methionine-labeled cellular proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Using ATCC strains as references, protein profile analysis showed that different Candida and other yeast species produced distinctively different patterns. Good agreement in results obtained with this approach and with other conventional systems was observed. Being accurate and reproducible, this approach provides a basis for the development of an alternative method for the identification of yeasts isolated from clinical specimens.

• Research Organization: Veterans General Hospital, Taipei, Taiwan
• OSTI ID: 6101683
• Journal Information: Anal. Biochem.; (United States), Vol. 175:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
POLYPEPTIDES
AUTORADIOGRAPHY
YEASTS
CLASSIFICATION
CANDIDA
ELECTROPHORESIS
METHIONINE
MOLECULAR WEIGHT
SULFUR 35
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DRUGS
EUMYCOTA
EVEN-ODD NUCLEI
FUNGI
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MICROORGANISMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
PLANTS
PROTEINS
RADIOISOTOPES
SULFUR ISOTOPES
550201* - Biochemistry- Tracer Techniques
550701 - Microbiology- Tracer Techniques