Transcription of a subset of human class II major histocompatibility complex genes is regulated by a nucleoprotein complex that contains c-fos or an antigenically related protein

Journal Article · Wed May 15 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.10.4304· OSTI ID:6097147

Ono, S J; Bazil, V; Strominger, J L ^[1]; Levi, B Z; Ozato, K ^[2]

---

1. Harvard Univ., Cambridge, MA (United States)
2. National Inst. of Health, Bethesda, MD (United States)

Transcriptional regulation of the human major histocompatibility complex class II genes requires at least two upstream elements, the X and Y boxes, located in the {minus}50- to {minus}150-base-pair region of all class II promoters. The DRA and DPB promoters contain phorbol ester-responsive elements overlapping the 3{prime} side of the X boxes. Mutation of this sequence down-regulates the efficiency of the DRA promoter, suggesting that a positive regulator(s) binds to this site. In this report, anti-sense c-fos RNA and an anti-c-fos antibody were used to show that the product of the protooncogene c-fos or an antigenically related protein is a component of a complex that binds to the X box and is required for maximal transcription from the DRA and DPB promoters. As c-fos (or its related proteins) cannot bind alone to DNA, these results suggest that it may dimerize with other members of the JUN/AP-1 family, such as hXBP1, to participate in the activation of a subset of class II major histocompatibility complex genes.

• OSTI ID: 6097147
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:10; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
TRANSCRIPTION
HISTOCOMPATIBILITY COMPLEX
GENE REGULATION
ENZYME IMMUNOASSAY
GENE MUTATIONS
HELA CELLS
LEUCINE
NUCLEOPROTEINS
ONCOGENES
PHORBOL ESTERS
PHOSPHORUS 32
PLASMIDS
AMINO ACIDS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
CARBOXYLIC ACIDS
CARCINOGENS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
ESTERS
IMMUNOASSAY
ISOTOPES
LIGHT NUCLEI
MUTATIONS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques