Binding and transepithelial transport of immunoglobulins by intestinal M cells: demonstration using monoclonal IgA antibodies against enteric viral proteins
M cells of intestinal epithelia overlying lymphoid follicles endocytose luminal macromolecules and microorganisms and deliver them to underlying lymphoid tissue. The effect of luminal secretory IgA antibodies on adherence and transepithelial transport of antigens and microorganisms by M cells is unknown. We have studied the interaction of monoclonal IgA antibodies directed against specific enteric viruses, or the hapten trinitrophenyl (TNP), with M cells. To produce monospecific IgA antibodies against mouse mammary tumor virus (MMTV) and reovirus type 1, Peyer's patch cells from mucosally immunized mice were fused with myeloma cells, generating hybridomas that secreted virus-specific IgA antibodies in monomeric and polymeric forms. One of two anti-MMTV IgA antibodies specifically bound the viral surface glycoprotein gp52, and 3 of 10 antireovirus IgA antibodies immunoprecipitated sigma 3 and mu lc surface proteins. 35S-labeled IgA antibodies injected intravenously into rats were recovered in bile as higher molecular weight species, suggesting that secretory component had been added on passage through the liver. Radiolabeled or colloidal gold-conjugated mouse IgA was injected into mouse, rat, and rabbit intestinal loops containing Peyer's patches. Light microscopic autoradiography and EM showed that all IgA antibodies (antivirus or anti-TNP) bound to M cell luminal membranes and were transported in vesicles across M cells. IgA-gold binding was inhibited by excess unlabeled IgA, indicating that binding was specific. IgG-gold also adhered to M cells and excess unlabeled IgG inhibited IgA-gold binding; thus binding was not isotype-specific. Immune complexes consisting of monoclonal anti-TNP IgA and TNP-ferritin adhered selectively to M cell membranes, while TNP-ferritin alone did not.
- Research Organization:
- Harvard Univ. Medical School, Boston, MA (USA)
- OSTI ID:
- 6080846
- Journal Information:
- J. Cell Biol.; (United States), Vol. 108:5
- Country of Publication:
- United States
- Language:
- English
Similar Records
B-lymphocyte differentiation in lethally irradiated and reconstituted mice. II. Recovery of humoral immune responsiveness. [X radiation]
Origin and differentiation of lymphocytes involved in the secretory IgA response
Related Subjects
IMMUNOGLOBULINS
MEMBRANE TRANSPORT
MONOCLONAL ANTIBODIES
BIOCHEMICAL REACTION KINETICS
ANTIGEN-ANTIBODY REACTIONS
AUTORADIOGRAPHY
CELL MEMBRANES
COLLOIDS
ELECTRON MICROSCOPY
EPITHELIUM
FLUORESCENCE
HYBRIDOMAS
LIVER
MICE
MOLECULAR WEIGHT
ONCOGENIC VIRUSES
PROTEINS
RABBITS
RATS
RECEPTORS
SPECIFICITY
SULFUR 35
TRACER TECHNIQUES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISPERSIONS
EVEN-ODD NUCLEI
GLANDS
GLOBULINS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LUMINESCENCE
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
MICROORGANISMS
MICROSCOPY
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PARASITES
RADIOISOTOPES
REACTION KINETICS
RODENTS
SULFUR ISOTOPES
TISSUES
VERTEBRATES
VIRUSES
550201* - Biochemistry- Tracer Techniques