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Title: Construction and evaluation of a regenerable flouroimmunochemical-based fiber optic biosensor

Abstract

A microscale fiber optic biosensor that is capable of in situ regeneration is described and characterized. By combining recently developed fiber optic sensing technology with a capillary column reagent delivery system, it is possible to perform a variety of benchtop affinity assay procedures repetitively and remotely. The configuration of the sensing chamber at the terminus of the fiber is an important design feature. The construction and operation of the sensor is described and the results of evaluations of the sensor using an antibody/antigen system are presented. Affinity assay steps such as the delivery of solid phase affinity reagents, secondary reagents, and rinse solutions are demonstrated. Sampling is accomplished by mild aspiration. Coefficients of variation (CVs) for these steps are all less than 10. The capability of selectivity measuring fluorescently-labeled anti-rabbit-immunoglobulin G (IgG), in the present of a similar protein, utilizing its immunospecific interaction with rabbit-IgG beads, is demonstrated and exhibits a CV of 6.2. A near linear calibration plot is presented over a concentration range of 0.011 mg ml{sup {minus}1} (approximately the limit of detection) to 0.11mg ml{sup {minus}1}. 30 refs., 4 figs., 2 tabs.

Authors:
; ;  [1];  [2];  [3]
  1. Tennessee Univ., Knoxville, TN (USA). Dept. of Chemistry
  2. Oak Ridge National Lab., TN (USA)
  3. Oberlin Coll., OH (USA). Dept. of Chemistry
Publication Date:
Research Org.:
Tennessee Univ., Knoxville, TN (USA). Dept. of Chemistry
Sponsoring Org.:
DOE/ER; National Science Foundation (NSF)
OSTI Identifier:
6077933
Report Number(s):
DOE/ER/13613-29
ON: DE91004886; CNN: CHE-8708581
DOE Contract Number:  
FG05-86ER13613
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 59 BASIC BIOLOGICAL SCIENCES; IMMUNOGLOBULINS; FLUORESCENCE; EXPERIMENTAL DATA; FIBER OPTICS; LASERS; RABBITS; ANIMALS; DATA; GLOBULINS; INFORMATION; LUMINESCENCE; MAMMALS; NUMERICAL DATA; ORGANIC COMPOUNDS; PROTEINS; VERTEBRATES; 440800* - Miscellaneous Instrumentation- (1990-); 550200 - Biochemistry

Citation Formats

Bowyer, J R, Alarie, J P, Sepaniak, M J, Vo-Dinh, Tuan, and Thompson, R Q. Construction and evaluation of a regenerable flouroimmunochemical-based fiber optic biosensor. United States: N. p., 1990. Web.
Bowyer, J R, Alarie, J P, Sepaniak, M J, Vo-Dinh, Tuan, & Thompson, R Q. Construction and evaluation of a regenerable flouroimmunochemical-based fiber optic biosensor. United States.
Bowyer, J R, Alarie, J P, Sepaniak, M J, Vo-Dinh, Tuan, and Thompson, R Q. Mon . "Construction and evaluation of a regenerable flouroimmunochemical-based fiber optic biosensor". United States.
@article{osti_6077933,
title = {Construction and evaluation of a regenerable flouroimmunochemical-based fiber optic biosensor},
author = {Bowyer, J R and Alarie, J P and Sepaniak, M J and Vo-Dinh, Tuan and Thompson, R Q},
abstractNote = {A microscale fiber optic biosensor that is capable of in situ regeneration is described and characterized. By combining recently developed fiber optic sensing technology with a capillary column reagent delivery system, it is possible to perform a variety of benchtop affinity assay procedures repetitively and remotely. The configuration of the sensing chamber at the terminus of the fiber is an important design feature. The construction and operation of the sensor is described and the results of evaluations of the sensor using an antibody/antigen system are presented. Affinity assay steps such as the delivery of solid phase affinity reagents, secondary reagents, and rinse solutions are demonstrated. Sampling is accomplished by mild aspiration. Coefficients of variation (CVs) for these steps are all less than 10. The capability of selectivity measuring fluorescently-labeled anti-rabbit-immunoglobulin G (IgG), in the present of a similar protein, utilizing its immunospecific interaction with rabbit-IgG beads, is demonstrated and exhibits a CV of 6.2. A near linear calibration plot is presented over a concentration range of 0.011 mg ml{sup {minus}1} (approximately the limit of detection) to 0.11mg ml{sup {minus}1}. 30 refs., 4 figs., 2 tabs.},
doi = {},
url = {https://www.osti.gov/biblio/6077933}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1990},
month = {1}
}

Technical Report:
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