Genomic clones for human cholinesterase
A human genomic library was prepared from peripheral white blood cells from a single donor by inserting an MboI partial digest into BamHI poly-linker sites of EMBL3. This library was screened using an oligolabeled human cholinesterase cDNA probe over 700 bp long. The latter probe was obtained from a human basal ganglia cDNA library. Of approximately 2 million clones screened with high stringency conditions several positive clones were identified; two have been plaque purified. One of these clones has been partially mapped using restriction enzymes known to cut within the coded region of the cDNA for human serum cholinesterase. Hybridization of the fragments and their sizes are as expected if the genomic clone is cholinesterase. Sequencing of the DNA fragments in M13 is in progress to verify the identify of the clone and the location of introns.
- Research Organization:
- Univ. of Michigan Medical School, Ann Arbor
- OSTI ID:
- 6076499
- Report Number(s):
- CONF-870644-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHOLINESTERASE
DNA-CLONING
BRAIN
DNA
DNA SEQUENCING
LEUKOCYTES
MAN
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOXYLESTERASES
CENTRAL NERVOUS SYSTEM
CLONING
ENZYMES
ESTERASES
HYDROLASES
MAMMALS
MATERIALS
NERVOUS SYSTEM
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550200* - Biochemistry