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Title: Dose-dependent changes in the antigenicity of bacterial endotoxin exposed to ionizing radiation. Report No. 2, 1986-1987

Abstract

The antigenic properties of the highly purified US reference standard endotoxin (RSE) exposed to varying doses of ionizing radiation were studied with double immuno-diffusion, immunoelectrophoresis, and immunoblotting. Rabbit RSE antisera identified 2 distinct major antigenic components for untreated RSE: one related to the O-polysaccharide side chain (O-antigenic specificity), the other to the R-core. Based on a serologic cross-reactivity of R-core of RSE (Escherichia coli 0113) with the R-core of the lipopolysaccharide from E. coli 0111, the core type of E. coli 0113 was identified as coli R3. Increasing exposure of RSE to ionizing radiation progressively destroyed all antigenic reactivities; at lower doses of radiation the rate of elimination differed for the 2 antigen classes. The O-polysaccharide was more sensitive to gamma radiation than the R-core and the O-antigenicity was lost before that of the R-core. Endotoxin molecules containing incomplete R-core (radiation-induced or mutant) did not react with the RSE antiserum. Keywords: Antigenicity, Reprints. (KT/KR)

Authors:
; ; ; ;
Publication Date:
Research Org.:
Naval Medical Research Inst., Bethesda, MD (USA)
OSTI Identifier:
6074336
Report Number(s):
AD-A-201399/3/XAB
Resource Type:
Technical Report
Resource Relation:
Other Information: Pub. in Jnl. of Clinical Laboratory Immunology, Vol. 24, 193-198(1987)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ANTIGENS; DOSE-RESPONSE RELATIONSHIPS; ENDOTOXINS; BIOLOGICAL RADIATION EFFECTS; ANTIBODIES; BACTERIA; ELECTROPHORESIS; ESCHERICHIA COLI; GAMMA RADIATION; IMMUNE SERUMS; IMMUNOLOGY; IONIZING RADIATIONS; MOLECULES; RABBITS; SENSITIVITY; ANIMALS; BIOLOGICAL EFFECTS; ELECTROMAGNETIC RADIATION; MAMMALS; MATERIALS; MICROORGANISMS; RADIATION EFFECTS; RADIATIONS; TOXIC MATERIALS; TOXINS; VERTEBRATES; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture

Citation Formats

Csako, G, Suba, E A, Tsai, C M, Mocca, L F, and Elin, R J. Dose-dependent changes in the antigenicity of bacterial endotoxin exposed to ionizing radiation. Report No. 2, 1986-1987. United States: N. p., 1987. Web.
Csako, G, Suba, E A, Tsai, C M, Mocca, L F, & Elin, R J. Dose-dependent changes in the antigenicity of bacterial endotoxin exposed to ionizing radiation. Report No. 2, 1986-1987. United States.
Csako, G, Suba, E A, Tsai, C M, Mocca, L F, and Elin, R J. 1987. "Dose-dependent changes in the antigenicity of bacterial endotoxin exposed to ionizing radiation. Report No. 2, 1986-1987". United States.
@article{osti_6074336,
title = {Dose-dependent changes in the antigenicity of bacterial endotoxin exposed to ionizing radiation. Report No. 2, 1986-1987},
author = {Csako, G and Suba, E A and Tsai, C M and Mocca, L F and Elin, R J},
abstractNote = {The antigenic properties of the highly purified US reference standard endotoxin (RSE) exposed to varying doses of ionizing radiation were studied with double immuno-diffusion, immunoelectrophoresis, and immunoblotting. Rabbit RSE antisera identified 2 distinct major antigenic components for untreated RSE: one related to the O-polysaccharide side chain (O-antigenic specificity), the other to the R-core. Based on a serologic cross-reactivity of R-core of RSE (Escherichia coli 0113) with the R-core of the lipopolysaccharide from E. coli 0111, the core type of E. coli 0113 was identified as coli R3. Increasing exposure of RSE to ionizing radiation progressively destroyed all antigenic reactivities; at lower doses of radiation the rate of elimination differed for the 2 antigen classes. The O-polysaccharide was more sensitive to gamma radiation than the R-core and the O-antigenicity was lost before that of the R-core. Endotoxin molecules containing incomplete R-core (radiation-induced or mutant) did not react with the RSE antiserum. Keywords: Antigenicity, Reprints. (KT/KR)},
doi = {},
url = {https://www.osti.gov/biblio/6074336}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1987},
month = {Thu Jan 01 00:00:00 EST 1987}
}

Technical Report:
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