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Title: Expression and sequences of T cell antigen receptor beta-chain genes in the thymus at an early stage after sublethal irradiation

Abstract

The sequential appearance of the thymocyte subpopulations and TCR gene messages occurred in the thymus of AKR mice (H-2k, Mlsa) from 7 to 14 days after sublethal irradiation. The thymocytes on day 7 after irradiation were composed of a large number of CD4+CD8+ blast-like cells and a relatively high proportion of CD4-CD8- cells (15 to 25%) but few CD3highCD4+CD8-/CD4-CD8+ cells. Approximately 22% of the CD4-CD8- cells were CD3high and -27% of the CD3highCD4-CD8- cells (-6% of whole CD4-CD8- cells) were F23.1+. The thymocytes on day 7 expressed a large amount of gamma- and delta-chain gene transcripts but reduced levels of alpha- and beta-chain gene transcripts. The V gene repertoire of 18 functional beta-chain cDNA derived from the thymocytes on day 7 was compared with those of 20 functional beta-chain cDNA derived from the thymocytes on day 14 which were composed of a large number of CD3lowCD4+CD8+ small-sized cells and a small number of CD3highCD4+CD8- cells. It is noteworthy that the distribution of V beta genes expressed in the thymocytes on day 7 was much the same as that in the thymocytes on day 14 but significantly different from that in normal BALB/c thymocytes as previously described. Interestingly, neither V betamore » 8.1 nor V beta 6 genes, which are important for recognition of the product of the Mlsa locus, was detected in these two cDNA libraries. These results suggest that clonal selection of TCR V beta repertoire, irrespective of positive or negative selection, appears to occur at the early stage of T cell differentiation, i.e., on the blast-like CD4+CD8+ thymocytes.« less

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Kyushu Univ., Fukuoka (Japan)
OSTI Identifier:
6073184
Resource Type:
Journal Article
Journal Name:
J. Immunol.; (United States)
Additional Journal Information:
Journal Volume: 142:10
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LYMPHOCYTES; CELL DIFFERENTIATION; THYMOCYTES; BIOLOGICAL RADIATION EFFECTS; THYMUS; GENE REGULATION; AMINO ACID SEQUENCE; ANTIGENS; DNA; GENES; IMMUNOGLOBULINS; MICE; PHENOTYPE; RADIATION CHIMERAS; RECEPTORS; SUBLETHAL IRRADIATION; ANIMAL CELLS; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CHIMERAS; CONNECTIVE TISSUE CELLS; GLOBULINS; IRRADIATION; LEUKOCYTES; LYMPHATIC SYSTEM; MAMMALS; MATERIALS; MEMBRANE PROTEINS; MOLECULAR STRUCTURE; MOSAICISM; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIATION EFFECTS; RODENTS; SOMATIC CELLS; VERTEBRATES; 560152* - Radiation Effects on Animals- Animals

Citation Formats

Yuuki, H, Yoshikai, Y, Kishihara, K, Matsuzaki, G, Ayukawa, K, and Nomoto, K. Expression and sequences of T cell antigen receptor beta-chain genes in the thymus at an early stage after sublethal irradiation. United States: N. p., 1989. Web.
Yuuki, H, Yoshikai, Y, Kishihara, K, Matsuzaki, G, Ayukawa, K, & Nomoto, K. Expression and sequences of T cell antigen receptor beta-chain genes in the thymus at an early stage after sublethal irradiation. United States.
Yuuki, H, Yoshikai, Y, Kishihara, K, Matsuzaki, G, Ayukawa, K, and Nomoto, K. 1989. "Expression and sequences of T cell antigen receptor beta-chain genes in the thymus at an early stage after sublethal irradiation". United States.
@article{osti_6073184,
title = {Expression and sequences of T cell antigen receptor beta-chain genes in the thymus at an early stage after sublethal irradiation},
author = {Yuuki, H and Yoshikai, Y and Kishihara, K and Matsuzaki, G and Ayukawa, K and Nomoto, K},
abstractNote = {The sequential appearance of the thymocyte subpopulations and TCR gene messages occurred in the thymus of AKR mice (H-2k, Mlsa) from 7 to 14 days after sublethal irradiation. The thymocytes on day 7 after irradiation were composed of a large number of CD4+CD8+ blast-like cells and a relatively high proportion of CD4-CD8- cells (15 to 25%) but few CD3highCD4+CD8-/CD4-CD8+ cells. Approximately 22% of the CD4-CD8- cells were CD3high and -27% of the CD3highCD4-CD8- cells (-6% of whole CD4-CD8- cells) were F23.1+. The thymocytes on day 7 expressed a large amount of gamma- and delta-chain gene transcripts but reduced levels of alpha- and beta-chain gene transcripts. The V gene repertoire of 18 functional beta-chain cDNA derived from the thymocytes on day 7 was compared with those of 20 functional beta-chain cDNA derived from the thymocytes on day 14 which were composed of a large number of CD3lowCD4+CD8+ small-sized cells and a small number of CD3highCD4+CD8- cells. It is noteworthy that the distribution of V beta genes expressed in the thymocytes on day 7 was much the same as that in the thymocytes on day 14 but significantly different from that in normal BALB/c thymocytes as previously described. Interestingly, neither V beta 8.1 nor V beta 6 genes, which are important for recognition of the product of the Mlsa locus, was detected in these two cDNA libraries. These results suggest that clonal selection of TCR V beta repertoire, irrespective of positive or negative selection, appears to occur at the early stage of T cell differentiation, i.e., on the blast-like CD4+CD8+ thymocytes.},
doi = {},
url = {https://www.osti.gov/biblio/6073184}, journal = {J. Immunol.; (United States)},
number = ,
volume = 142:10,
place = {United States},
year = {Mon May 15 00:00:00 EDT 1989},
month = {Mon May 15 00:00:00 EDT 1989}
}