Modulation of tyrosine hydroxylase gene expression in the central nervous system visualized by in situ hybridization
cDNA probe was used for in situ hybridization studies on histological sections through the locus coeruleus, substantia nigra, and the ventral tegmental area of the rat brain. Experimental conditions were established that yielded no background and no signal when pBR322 was used as control probe. Using the tyrosine hydroxylase probe, the authors ascertained the specificity of the labeling over catecholaminergic cells by denervation experiments and comparison of the hybridization pattern with that of immunoreactivity. The use of /sup 35/S-labeled probe enabled the hybridization signal to be resolved at the cellular level. A single injection of reserpine into the rat led to an increase of the intensity of the autoradiographic signal over the locus coeruleus area, confirming an RNA gel blot analysis. The potential of in situ hybridization to analyze patterns of modulation of gene activity as a result of nervous activity is discussed.
- Research Organization:
- Institut National de la Sante et de la Recherche Medicale, Saint-Genis-Laval, France
- OSTI ID:
- 6069706
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:6
- Country of Publication:
- United States
- Language:
- English
Similar Records
Hybrid histochemical localization of tyrosine hydroxylase mRNA in the CNS
Autoradiographic distribution of /sup 125/I-galanin binding sites in the rat central nervous system
Related Subjects
BRAIN
AUTORADIOGRAPHY
HYDROXYLASES
GENE REGULATION
RECOMBINANT DNA
TISSUE DISTRIBUTION
CYTOCHEMISTRY
HYBRIDIZATION
RATS
SULFUR 35
TYROSINE
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DNA
ENZYMES
EVEN-ODD NUCLEI
HYDROXY ACIDS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
RADIOISOTOPES
RODENTS
SULFUR ISOTOPES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics