Expression of the O6-methylguanine-DNA methyltransferase gene MGMT in MER+ and MER- human tumor cells

Journal Article · Sat Dec 15 00:00:00 EST 1990 · Cancer Research; (USA)

OSTI ID:6065082

Fornace, Jr, A J; Papathanasiou, M A; Hollander, M C; Yarosh, D B ^[1]

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1. National Cancer Institute, Bethesda, MD (USA)

DNA probes prepared from human O6-methylguanine-DNA methyltransferase complementary DNA were hybridized to mRNA isolated from human liver and fifteen human tumor cell lines proficient (Mer+) or deficient (Mer-) in transferase activity. Liver and Mer+ cells contained levels of transferase-specific mRNA that correlated with their transferase activity levels, whereas Mer- cells contained undetectable amounts of transferase mRNA. The mRNA levels were not induced in human cells by treatments that induce other DNA damage-inducible genes. These results demonstrate that in human cells the transferase gene is constitutively expressed, that its expression is related to activity levels, and that in Mer- tumor cells the expression of the transferase gene is probably blocked at the level of mRNA production.

• OSTI ID: 6065082
• Journal Information: Cancer Research; (USA), Vol. 50:24; ISSN 0008-5472
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
METHYL TRANSFERASES
GENE REGULATION
DNA HYBRIDIZATION
LIVER
MAN
MESSENGER-RNA
METHYL METHANESULFONATE
NITROSO COMPOUNDS
PHENOTYPE
TUMOR CELLS
X RADIATION
ANIMAL CELLS
ANIMALS
BODY
DIGESTIVE SYSTEM
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERS
GLANDS
HYBRIDIZATION
IONIZING RADIATIONS
MAMMALS
MUTAGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PRIMATES
RADIATIONS
RNA
SULFONIC ACID ESTERS
TRANSFERASES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550200 - Biochemistry