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Title: Multiplexed fluorescence detector for capillary electrophoresis using axial optical fiber illumination

Abstract

Recently, we developed an axial beam excitation scheme for capillary electrophoresis. We report the use of this excitation geometry to simultaneously monitor 10 capillary tubes undergoing electrophoresis. This represents a truly parallel multiplexing scheme for monitoring large arrays of capillaries. The system has no moving parts. Data rates are compatible with state-of-the-art run times in CGE and are fast enough even if there is another order of magnitude increase in DNA sequencing rates per lane in the future. 24 refs., 3 figs.

Authors:
;  [1]
  1. Iowa State Univ., Ames (United States)
Publication Date:
OSTI Identifier:
6062895
DOE Contract Number:  
W-7405-ENG-82
Resource Type:
Journal Article
Journal Name:
Analytical Chemistry (Washington); (United States)
Additional Journal Information:
Journal Volume: 65:7; Journal ID: ISSN 0003-2700
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; DNA; DETECTION; CAPILLARIES; CHROMOSOMES; DNA SEQUENCING; ELECTROPHORESIS; EXCITATION; FLUORESCENCE; MULTIPLEXERS; OPTICAL FIBERS; BLOOD VESSELS; BODY; CARDIOVASCULAR SYSTEM; ELECTRONIC EQUIPMENT; ENERGY-LEVEL TRANSITIONS; EQUIPMENT; FIBERS; LUMINESCENCE; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANS; STRUCTURAL CHEMICAL ANALYSIS; 550200* - Biochemistry; 400100 - Analytical & Separations Chemistry

Citation Formats

Taylor, J A, and Yeung, E A. Multiplexed fluorescence detector for capillary electrophoresis using axial optical fiber illumination. United States: N. p., 1993. Web. doi:10.1021/ac00055a021.
Taylor, J A, & Yeung, E A. Multiplexed fluorescence detector for capillary electrophoresis using axial optical fiber illumination. United States. https://doi.org/10.1021/ac00055a021
Taylor, J A, and Yeung, E A. Thu . "Multiplexed fluorescence detector for capillary electrophoresis using axial optical fiber illumination". United States. https://doi.org/10.1021/ac00055a021.
@article{osti_6062895,
title = {Multiplexed fluorescence detector for capillary electrophoresis using axial optical fiber illumination},
author = {Taylor, J A and Yeung, E A},
abstractNote = {Recently, we developed an axial beam excitation scheme for capillary electrophoresis. We report the use of this excitation geometry to simultaneously monitor 10 capillary tubes undergoing electrophoresis. This represents a truly parallel multiplexing scheme for monitoring large arrays of capillaries. The system has no moving parts. Data rates are compatible with state-of-the-art run times in CGE and are fast enough even if there is another order of magnitude increase in DNA sequencing rates per lane in the future. 24 refs., 3 figs.},
doi = {10.1021/ac00055a021},
url = {https://www.osti.gov/biblio/6062895}, journal = {Analytical Chemistry (Washington); (United States)},
issn = {0003-2700},
number = ,
volume = 65:7,
place = {United States},
year = {1993},
month = {4}
}