Characterization and mapping of a nonessential pseudorabies virus glycoprotein

Journal Article · Tue Apr 01 00:00:00 EST 1986 · J. Virol.; (United States)

OSTI ID:6046342

Wathen, M W; Wathen, L M.K.

Antigenic variants of pseudorabies virus (PRV) containing mutations in a viral glycoprotein with a molecular weight of 82,000 (gIII) were isolated by selecting for resistance to a complement-dependent neutralizing monoclonal antibody (MCA82-2) directed against gIII. These mutants were completely resistant to neutralization with MCA82-2 in the presence of complement. Two mutants selected for further studies either did not express gIII or expressed an improperly processed form of the glycoproteins. The mutations were also associated with an altered plaque morphology (syncytium formation). The gIII gene was mapped by the marker rescue of a gIII/sup -/ mutant with cloned restriction enzyme fragments to the long unique region of the PRV genome between 0.376 and 0.383 map units. This corresponds to the map location of a glycoprotein described by Robbins et al. Since gIII is nonessential for viral replication in cell culture and has several other characteristics in common with the herpes simplex virus glycoprotein gC, gIII may represent the PRV equivalent to herpes simplex virus gC.

• Research Organization: Department of Agriculture, Ames, IA (USA)
• OSTI ID: 6046342
• Journal Information: J. Virol.; (United States), Vol. 58:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOPROTEINS
GENETIC MAPPING
RABIES
PATHOGENESIS
CARBON 14 COMPOUNDS
COMPLEMENT
ELECTROPHORESIS
GLUCOSAMINE
HERPES SIMPLEX
MOLECULAR WEIGHT
MONOCLONAL ANTIBODIES
MUTATIONS
VIRUSES
AMINES
ANTIBODIES
CARBOHYDRATES
DISEASES
GLYCOPROTEINS
HEXOSAMINES
HEXOSES
INFECTIOUS DISEASES
LABELLED COMPOUNDS
MAPPING
MICROORGANISMS
MONOSACCHARIDES
NERVOUS SYSTEM DISEASES
ORGANIC COMPOUNDS
PARASITES
PROTEINS
SACCHARIDES
SKIN DISEASES
VIRAL DISEASES
550701* - Microbiology- Tracer Techniques