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Title: Immunoreactive somatomedin A in human serum

Abstract

A RIA has been developed for somatomedin A (SM-A) utilizing Sepharose-bound antibodies. This assay, measuring SM-A, the insulin-like growth factors 1 and 2, and somatomedin C, allows determination in serum samples. In comparison with a serum standard, the mean serum levels in patients with acromegaly or GH deficiency and healthy subjects were 8.7 +- 0.7 (n=25), 0.24 +- 0.02 (n=25), and 1.15 +- 0.11 U/ml, respectively. The correlation coefficient between immunoreactive SM-A and SM-A by radioreceptor assay was highly significant (r=0.93), although the potency ratio of SM-A between the two groups of patients was higher in the RIA than in the radioreceptor assay. Gel chromatography revealed that SM-A in acromegalic serum is bound to a carrier protein which is absent in patients with GH deficiency. After gel chromatography at low pH, 90% of applied immunoreactive SM-A was recovered in the low molecular weight fraction and consisted mainly of neutral polypeptides.

Authors:
; ; ;
Publication Date:
Research Org.:
Karolinska Hospital, Stockholm, Sweden
OSTI Identifier:
6037161
Resource Type:
Journal Article
Journal Name:
J. Clin. Endocrinol. Metab.; (United States)
Additional Journal Information:
Journal Volume: 48:2
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; HORMONES; RADIOIMMUNOASSAY; ACROMEGALY; ANTIBODIES; BLOOD SERUM; CHROMATOGRAPHY; EXPERIMENTAL DATA; GRAPHS; MAN; PATIENTS; ANIMALS; DATA; DATA FORMS; DISEASES; ENDOCRINE DISEASES; INFORMATION; ISOTOPE APPLICATIONS; MAMMALS; NUMERICAL DATA; PRIMATES; RADIOASSAY; SEPARATION PROCESSES; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Hall, K., Brandt, J., Enberg, G., and Fryklund, L. Immunoreactive somatomedin A in human serum. United States: N. p., 1979. Web. doi:10.1210/jcem-48-2-271.
Hall, K., Brandt, J., Enberg, G., & Fryklund, L. Immunoreactive somatomedin A in human serum. United States. doi:10.1210/jcem-48-2-271.
Hall, K., Brandt, J., Enberg, G., and Fryklund, L. Thu . "Immunoreactive somatomedin A in human serum". United States. doi:10.1210/jcem-48-2-271.
@article{osti_6037161,
title = {Immunoreactive somatomedin A in human serum},
author = {Hall, K. and Brandt, J. and Enberg, G. and Fryklund, L.},
abstractNote = {A RIA has been developed for somatomedin A (SM-A) utilizing Sepharose-bound antibodies. This assay, measuring SM-A, the insulin-like growth factors 1 and 2, and somatomedin C, allows determination in serum samples. In comparison with a serum standard, the mean serum levels in patients with acromegaly or GH deficiency and healthy subjects were 8.7 +- 0.7 (n=25), 0.24 +- 0.02 (n=25), and 1.15 +- 0.11 U/ml, respectively. The correlation coefficient between immunoreactive SM-A and SM-A by radioreceptor assay was highly significant (r=0.93), although the potency ratio of SM-A between the two groups of patients was higher in the RIA than in the radioreceptor assay. Gel chromatography revealed that SM-A in acromegalic serum is bound to a carrier protein which is absent in patients with GH deficiency. After gel chromatography at low pH, 90% of applied immunoreactive SM-A was recovered in the low molecular weight fraction and consisted mainly of neutral polypeptides.},
doi = {10.1210/jcem-48-2-271},
journal = {J. Clin. Endocrinol. Metab.; (United States)},
number = ,
volume = 48:2,
place = {United States},
year = {1979},
month = {2}
}