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Title: Dried Staphylococcus aureus as a rapid immunological separating agent in radioimmunoassays

Abstract

A rapid (less than or equal to 60 seconds) immunological separation of antigen-antibody complexes from free antigen has been developed in radioimmunoassays (RIAs) of luteinizing hormone (LH), follicle stimulating hormone (FSH), prolactin (PRL) and ..beta..-endorphin by using suspensions of dried Staphylococcus aureus rich in protein-A. In the systems tested parallel dose-response curves were obtained for protein-A and second antibody precipitations. The sensitivity of the protein-A method is equal to or higher than that of second antibody method. Tissue culture medium and serum hormone levels measured with RIAs using protein-A are similar to those detected with double antibody methods. The technique may be of general use in all RIAs utilizing antisera from species whose IgG are known to be bound by protein-A.

Authors:
;
Publication Date:
Research Org.:
Salk Inst. for Biological Studies, La Jolla, CA
OSTI Identifier:
6037157
Resource Type:
Journal Article
Journal Name:
J. Clin. Endocrinol. Metab.; (United States)
Additional Journal Information:
Journal Volume: 48:2
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ENKEPHALINS; RADIOIMMUNOASSAY; FSH; LH; LTH; ANTIBODIES; ANTIGENS; EXPERIMENTAL DATA; GRAPHS; ISOLATED VALUES; STAPHYLOCOCCUS; BACTERIA; DATA; DATA FORMS; GONADOTROPINS; HORMONES; INFORMATION; ISOTOPE APPLICATIONS; MICROORGANISMS; NUMERICAL DATA; ORGANIC COMPOUNDS; PEPTIDE HORMONES; PEPTIDES; PITUITARY HORMONES; POLYPEPTIDES; PROTEINS; RADIOASSAY; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Ying, S.Y., and Guillemin, R. Dried Staphylococcus aureus as a rapid immunological separating agent in radioimmunoassays. United States: N. p., 1979. Web. doi:10.1210/jcem-48-2-360.
Ying, S.Y., & Guillemin, R. Dried Staphylococcus aureus as a rapid immunological separating agent in radioimmunoassays. United States. doi:10.1210/jcem-48-2-360.
Ying, S.Y., and Guillemin, R. Thu . "Dried Staphylococcus aureus as a rapid immunological separating agent in radioimmunoassays". United States. doi:10.1210/jcem-48-2-360.
@article{osti_6037157,
title = {Dried Staphylococcus aureus as a rapid immunological separating agent in radioimmunoassays},
author = {Ying, S.Y. and Guillemin, R.},
abstractNote = {A rapid (less than or equal to 60 seconds) immunological separation of antigen-antibody complexes from free antigen has been developed in radioimmunoassays (RIAs) of luteinizing hormone (LH), follicle stimulating hormone (FSH), prolactin (PRL) and ..beta..-endorphin by using suspensions of dried Staphylococcus aureus rich in protein-A. In the systems tested parallel dose-response curves were obtained for protein-A and second antibody precipitations. The sensitivity of the protein-A method is equal to or higher than that of second antibody method. Tissue culture medium and serum hormone levels measured with RIAs using protein-A are similar to those detected with double antibody methods. The technique may be of general use in all RIAs utilizing antisera from species whose IgG are known to be bound by protein-A.},
doi = {10.1210/jcem-48-2-360},
journal = {J. Clin. Endocrinol. Metab.; (United States)},
number = ,
volume = 48:2,
place = {United States},
year = {1979},
month = {2}
}