Protein microcrystal diffraction and the effects of radiation damage with ultra-high-flux synchrotron radiation

Hedman, B; Hodgson, K O; Helliwell, J R; Liddington, R; Papiz, M Z

doi:10.1073/pnas.82.22.7604

Title: Protein microcrystal diffraction and the effects of radiation damage with ultra-high-flux synchrotron radiation

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Abstract

By using ultra-high-flux synchrotron x-radiation from a wiggler source, good Laue diffraction data have been obtained from protein microcrystals of size 30 x 35 x 10 ..mu..m/sup 3/, mounted wet in glass capillaries. At the flux level of 10/sup 13/-10/sup 14/ photons per sec/mm/sup 2/, the radiation damage is still low enough to allow a large survey of reciprocal space for a microcrystal and a complete survey for a normal-sized protein crystal. The development of sources for ultra-high intensity synchrotron radiation is thus an important improvement in the technique for determination of structure through protein crystallography as well as in other cases where crystal size is often a limiting factor.

Authors:: Hedman, B; Hodgson, K O; Helliwell, J R; Liddington, R; Papiz, M Z

Publication Date:: Fri Nov 01 00:00:00 EST 1985

Research Org.:: Stanford Linear Accelerator Center, CA

OSTI Identifier:: 6035183

Resource Type:: Journal Article

Journal Name:: Proc. Natl. Acad. Sci. U.S.A.; (United States)

Additional Journal Information:: Journal Volume: 82:22

Country of Publication:: United States

Language:: English

Subject:: 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; PROTEINS; BIOLOGICAL RADIATION EFFECTS; CRYSTALS; PARTICLE SIZE; SYNCHROTRON RADIATION; SYNCHROTRON RADIATION SOURCES; X RADIATION; X-RAY DIFFRACTION; BIOLOGICAL EFFECTS; BREMSSTRAHLUNG; COHERENT SCATTERING; DIFFRACTION; ELECTROMAGNETIC RADIATION; IONIZING RADIATIONS; ORGANIC COMPOUNDS; RADIATION EFFECTS; RADIATION SOURCES; RADIATIONS; SCATTERING; SIZE; 560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)

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                    Hedman, B, Hodgson, K O, Helliwell, J R, Liddington, R, and Papiz, M Z. Protein microcrystal diffraction and the effects of radiation damage with ultra-high-flux synchrotron radiation.  United States: N. p., 1985. 
        Web.  doi:10.1073/pnas.82.22.7604.

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                    Hedman, B, Hodgson, K O, Helliwell, J R, Liddington, R, & Papiz, M Z. Protein microcrystal diffraction and the effects of radiation damage with ultra-high-flux synchrotron radiation.  United States.  https://doi.org/10.1073/pnas.82.22.7604

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                    Hedman, B, Hodgson, K O, Helliwell, J R, Liddington, R, and Papiz, M Z. 1985.  
        "Protein microcrystal diffraction and the effects of radiation damage with ultra-high-flux synchrotron radiation".  United States.  https://doi.org/10.1073/pnas.82.22.7604.

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@article{osti_6035183,

  title        = {Protein microcrystal diffraction and the effects of radiation damage with ultra-high-flux synchrotron radiation},

  author       = {Hedman, B and Hodgson, K O and Helliwell, J R and Liddington, R and Papiz, M Z},

  abstractNote = {By using ultra-high-flux synchrotron x-radiation from a wiggler source, good Laue diffraction data have been obtained from protein microcrystals of size 30 x 35 x 10 ..mu..m/sup 3/, mounted wet in glass capillaries. At the flux level of 10/sup 13/-10/sup 14/ photons per sec/mm/sup 2/, the radiation damage is still low enough to allow a large survey of reciprocal space for a microcrystal and a complete survey for a normal-sized protein crystal. The development of sources for ultra-high intensity synchrotron radiation is thus an important improvement in the technique for determination of structure through protein crystallography as well as in other cases where crystal size is often a limiting factor.},

  doi          = {10.1073/pnas.82.22.7604},

  url          = {https://www.osti.gov/biblio/6035183},
  journal      = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number       = ,

  volume       = 82:22,

  place        = {United States},

  year         = {Fri Nov 01 00:00:00 EST 1985},

  month        = {Fri Nov 01 00:00:00 EST 1985}

}

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