Control of adenovirus early gene expression: Posttranscriptional control mediated by both viral and cellular gene products
An adenovirus type 5 host range mutant (hr-1) located in region E1A and phenotypically defective in expressing viral messenger ribonucleic acid (RNA) from other early regions was analyzed for accumulation of viral RNA in the presence of protein synthesis inhibitors. Nuclear RNA was transcribed from all early regions at the same rate, regardless of whether the drug was present or absent. As expected, low or undetectable levels of RNA were found in the cytoplasm of hr-1-infected cells compared with the wild-type adenovirus type 5 in the absence of drug. When anisomycin was added 30 min before hr-1 infection, cytoplasmic RNA was abundant from early regions E3 and E4 when assayed by filter hybridization. In accordance, early regions E3 and E4 viral messenger RNA species were detected by the S1 endonuclease mapping technique only in hr-1-infected cells that were treated with the drug. Similar results were obtained by in vitro translation studies. Together, these results suggest that this adenovirus type 5 mutant lacks a viral gene product necessary for accumulation of viral messenger RNA, but not for transcription. It is proposed that a cellular gene product serves as a negative regulator of viral messenger RNA accumulation at the posttranscriptional level.
- Research Organization:
- Dept. of Microbiology, The Biomedical Center, S-751 23 Uppsala
- OSTI ID:
- 6030063
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 1:9
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ADENOVIRUS
CLASSIFICATION
GENE REGULATION
HOST-CELL REACTIVATION
INFECTIVITY
POST-TRANSLATION MODIFICATION
MESSENGER-RNA
BIOLOGICAL FUNCTIONS
DEFECTS
RNA-ASE
ENZYME INHIBITORS
GENE REPRESSORS
TRANSCRIPTION
BIOLOGICAL ACCUMULATION
DRUGS
HYBRIDIZATION
PHENOTYPE
PROTEINS
RECOMBINANT DNA
TOXICITY
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA
ENZYMES
ESTERASES
FUNCTIONS
HYDROLASES
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOPROTEINS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
RECOVERY
REPAIR
RNA
VIRUSES
550400* - Genetics
550200 - Biochemistry