Metabolic characteristics of skeletal muscle from lean and obese Zucker rats
The purpose of this study was to determine if the metabolic response to obesity and to pair feeding of obese Zucker rats to lean Zucker rats was similar across skeletal muscles. Oxidation of glucose, palmitate and isoleucine was studied in muscle strips in vitro using appropriate /sup 14 -/carbon substrates as tracers. The plantaris muscle was subjected to histochemical analyses using an alkaline actomyosin ATPase, NADH-tetrazolium reductase and an oil red 0 stain. Soleus muscles from both ad libitum and pair fed obese rats oxidized less glucose to CO/sub 2/, but released similar amounts of lactate when compared to the soleus muscles of lean rats. Oxidation of glucose was similar in the extensor digitorum longus (EDL) muscle of ad libitum fed obese rats, but lower when pair fed to the intake of lean rats. No differences were apparent in palmitate oxidation to CO/sub 2/ or in incorporation into lipid, except in the EDL muscle of pair-fed obese rats which exhibited a higher rate for palmitate metabolism when compared with lean rats. Isoleucine oxidation to CO/sub 2/ was higher in the EDL and plantaris muscles, but similar in the soleus muscle of ad libitum-fed obese rats when compared with lean rats. The magnitude of the difference in isoleucine oxidation was similar when the obese rats were pair fed. No differences in the percentage of plantaris muscle fibers sensitive to alkaline ATPase staining were observed. The plantaris muscle of obese rats, contained a higher proportion of oxidative fibers. These results indicate the great risk in generalizing about metabolic activity of the whole skeletal muscle mass based on observations made on one, or even two, distinct muscles in this animal model. Also, pair feeding of obese to lean Zucker rats did not result in uniform change sin metabolism between muscles of the obese rats.
- Research Organization:
- Richard B. Russell Research Center, Athens, GA (USA)
- OSTI ID:
- 6029558
- Journal Information:
- Growth; (United States), Vol. 51:4
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effects of dehydroepiandrosterone (DHEA) on glucose metabolism in isolated hepatocytes from Zucker rats
Evidence for increased peroxidative activity in muscles from streptozotocin-diabetic rats
Related Subjects
GLUCOSE
METABOLISM
HEXADECANOIC ACID
LEUCINE
MUSCLES
ADIPOSE TISSUE
ATP-ASE
BIOLOGICAL MODELS
CARBON 14 COMPOUNDS
CARBON DIOXIDE
IN VITRO
OXIDOREDUCTASES
RATS
TRACER TECHNIQUES
ACID ANHYDRASES
ALDEHYDES
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
BODY
CARBOHYDRATES
CARBON COMPOUNDS
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
CONNECTIVE TISSUE
ENZYMES
HEXOSES
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PHOSPHOHYDROLASES
RODENTS
SACCHARIDES
TISSUES
VERTEBRATES
550501* - Metabolism- Tracer Techniques