skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: /sup 14/CO/sub 2/ pulse-/sup 12/CO/sub 2/ chase analyses of C/sub 4/-acid metabolism in C/sub 3/-C/sub 4/ intermediate species of Flaveria at the CO/sub 2/ compensation concentration (r). [Flaveria floridana; Flaveria anomala]

Abstract

Photosynthetic C/sub 4/-acid metabolism in leaves of C/sub 3/-C/sub 4/ intermediate Flaveria species was examined by /sup 14/CO/sub 2/ pulse-/sup 12/CO/sub 2/ chase experiments conducted at external CO/sub 2/-levels approximating air and ..gamma... Analysis of the percent distribution of /sup 14/C after a 10-s pulse showed an enhanced labeling of malate and aspartate at ..gamma.. in the C/sub 3/-C/sub 4/ species. This stimulation of /sup 14/CO/sub 2/ fixation by PEP carboxylase ranged from 1.7-(F. floridana) to 3.6-fold (F. anomala). A /sup 12/CO/sub 2/-chase at ..gamma.. revealed a significant turnover of C/sub 4/ acids for only F. floridana. C/sub 4/-acid labeling in C/sub 3/ and C/sub 4/ Flaveria species was relatively unresponsive to changes in pCO/sub 2/. These data imply that the C/sub 3/-C/sub 4/ intermediate Flaveria species with less advanced C/sub 4/ attributes have a greater capacity for increased CO/sub 2/ fixation via PEP carboxylase at ..gamma.. versus air. Thus, labeling of C/sub 3/-C/sub 4/ leaves at ..sigma.. may be an effective tool for assessing the degree of true C/sub 4/-photosynthesis as well as the potential mechanism involved in reducing photorespiration.

Authors:
;
Publication Date:
Research Org.:
Univ. of Nebraska, Lincoln
OSTI Identifier:
6028154
Report Number(s):
CONF-8707108-
Journal ID: CODEN: PPYSA; TRN: 87-037214
Resource Type:
Conference
Journal Name:
Plant Physiol., Suppl.; (United States)
Additional Journal Information:
Journal Volume: 83:4; Conference: Annual meeting of the American Society of Plant Physiologists, St. Louis, MO, USA, 19 Jul 1987
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; C4 SPECIES; PHOTOSYNTHESIS; CARBOXYLIC ACIDS; METABOLISM; ASPARTIC ACID; CARBON 14 COMPOUNDS; CARBON DIOXIDE; CARBON DIOXIDE FIXATION; LEAVES; MALIC ACID; RESPIRATION; TRACER TECHNIQUES; AMINO ACIDS; CARBON COMPOUNDS; CARBON OXIDES; CHALCOGENIDES; CHEMICAL REACTIONS; HYDROXY ACIDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; OXIDES; OXYGEN COMPOUNDS; PHOTOCHEMICAL REACTIONS; SYNTHESIS; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Chastain, C J, and Chollet, R. /sup 14/CO/sub 2/ pulse-/sup 12/CO/sub 2/ chase analyses of C/sub 4/-acid metabolism in C/sub 3/-C/sub 4/ intermediate species of Flaveria at the CO/sub 2/ compensation concentration (r). [Flaveria floridana; Flaveria anomala]. United States: N. p., 1987. Web.
Chastain, C J, & Chollet, R. /sup 14/CO/sub 2/ pulse-/sup 12/CO/sub 2/ chase analyses of C/sub 4/-acid metabolism in C/sub 3/-C/sub 4/ intermediate species of Flaveria at the CO/sub 2/ compensation concentration (r). [Flaveria floridana; Flaveria anomala]. United States.
Chastain, C J, and Chollet, R. 1987. "/sup 14/CO/sub 2/ pulse-/sup 12/CO/sub 2/ chase analyses of C/sub 4/-acid metabolism in C/sub 3/-C/sub 4/ intermediate species of Flaveria at the CO/sub 2/ compensation concentration (r). [Flaveria floridana; Flaveria anomala]". United States.
@article{osti_6028154,
title = {/sup 14/CO/sub 2/ pulse-/sup 12/CO/sub 2/ chase analyses of C/sub 4/-acid metabolism in C/sub 3/-C/sub 4/ intermediate species of Flaveria at the CO/sub 2/ compensation concentration (r). [Flaveria floridana; Flaveria anomala]},
author = {Chastain, C J and Chollet, R},
abstractNote = {Photosynthetic C/sub 4/-acid metabolism in leaves of C/sub 3/-C/sub 4/ intermediate Flaveria species was examined by /sup 14/CO/sub 2/ pulse-/sup 12/CO/sub 2/ chase experiments conducted at external CO/sub 2/-levels approximating air and ..gamma... Analysis of the percent distribution of /sup 14/C after a 10-s pulse showed an enhanced labeling of malate and aspartate at ..gamma.. in the C/sub 3/-C/sub 4/ species. This stimulation of /sup 14/CO/sub 2/ fixation by PEP carboxylase ranged from 1.7-(F. floridana) to 3.6-fold (F. anomala). A /sup 12/CO/sub 2/-chase at ..gamma.. revealed a significant turnover of C/sub 4/ acids for only F. floridana. C/sub 4/-acid labeling in C/sub 3/ and C/sub 4/ Flaveria species was relatively unresponsive to changes in pCO/sub 2/. These data imply that the C/sub 3/-C/sub 4/ intermediate Flaveria species with less advanced C/sub 4/ attributes have a greater capacity for increased CO/sub 2/ fixation via PEP carboxylase at ..gamma.. versus air. Thus, labeling of C/sub 3/-C/sub 4/ leaves at ..sigma.. may be an effective tool for assessing the degree of true C/sub 4/-photosynthesis as well as the potential mechanism involved in reducing photorespiration.},
doi = {},
url = {https://www.osti.gov/biblio/6028154}, journal = {Plant Physiol., Suppl.; (United States)},
number = ,
volume = 83:4,
place = {United States},
year = {Wed Apr 01 00:00:00 EST 1987},
month = {Wed Apr 01 00:00:00 EST 1987}
}

Conference:
Other availability
Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that hold this conference proceeding.

Save / Share: