Active oxygen acts as a promoter of transformation in mouse embryo C3H/10T1/2/C18 fibroblasts
There is much evidence from in vivo and in vitro carcinogenesis studies that active oxygen species play a role in tumor promotion. We tested directly whether superoxide produced extracellularly by xanthine-xanthine oxidase (X-XO) has the capacity to promote initiated mouse embryo C3H/10T1/2 fibroblasts. Cell cultures initiated with either /sup 137/Cs ..gamma..-rays or benzo(a)pyrene diol epoxide I were found to transform 3-30 times more effectively when subsequently treated daily for 3 weeks with nontoxic doses of X-XO. Scavengers of active oxygen radicals such as superoxide dismutase or superoxide dismutase in combination with catalase reduced the frequency of appearance of transformed foci by 3-25 times when compared to cultures receiving X-XO alone. These results show that active oxygen species such as superoxide and H/sub 2/O/sub 2/ can act in a promotional manner that mimics the effects of the mouse skin promoter phorbol 12-myristate 13-acetate in this system. X-XO also acted as a weak complete carcinogen. 27 references, 4 figures, 1 table.
- Research Organization:
- Swiss Inst. for Experimental Cancer Research, Lausanne
- OSTI ID:
- 6027212
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 81:7
- Country of Publication:
- United States
- Language:
- English
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EPOXIDES
TOXICITY
FIBROBLASTS
BIOLOGICAL RADIATION EFFECTS
SUPEROXIDE DISMUTASE
ENZYME ACTIVITY
CARCINOGENS
CELL CULTURES
CESIUM 137
EMBRYOS
GAMMA RADIATION
LABELLED COMPOUNDS
MICE
ONCOGENIC TRANSFORMATIONS
TRACER TECHNIQUES
TUMOR PROMOTERS
XANTHINES
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMALS
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CESIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
ENZYMES
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDOREDUCTASES
PROMOTERS
PURINES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
560152 - Radiation Effects on Animals- Animals