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Title: Synthesis and uptake of cytoplasmically synthesized pyruvate, Pi dikinase polypeptide by chloroplasts

Abstract

Polyadenylated RNA was isolated from maize leaves and translated in vitro. In agreement with a previous report by others, the authors found among the translation products a 110-kilodalton pyruvate orthophosphate dikinase (PPDK) precursor that is about 16 kilodaltons larger than the polypeptide isolated from cells. This maize PPDK precursor polypeptide was taken up from the translation product mixture by intact spinach chloroplasts and yielded a mature PPDK polypeptide (94 kilodaltons). The uptake and processing support the proposal that the extra 16-kilodalton size of the polypeptide from in vitro translation of maize leaf mRNA represents a transit sequence which is cleaved after its entry into chloroplasts. Moreover, these results provide additional evidence that in vivo in maize leaf cells PPDK polypeptide is synthesized in the cytoplasm and is transported into the chloroplasts. Location of PPDK in C/sub 3/ plant leaves was investigated by immunochemical analysis. A protein blot of stromal protein in each case gave rise to bands corresponding to authentic PPDK polypeptide. This result indicates that PPDK is present in chloroplasts of C/sub 3/ plant leaves as it is in the case of C/sub 4/ plants. 35 references, 3 figures.

Authors:
;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
OSTI Identifier:
6026264
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Journal Article
Journal Name:
Plant Physiol.; (United States)
Additional Journal Information:
Journal Volume: 78:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PHOSPHOTRANSFERASES; BIOSYNTHESIS; UPTAKE; CHLOROPLASTS; ENZYME IMMUNOASSAY; LEAVES; MAIZE; MESSENGER-RNA; POLYPEPTIDES; PYRUVIC ACID; SPINACH; WHEAT; CARBOXYLIC ACIDS; CELL CONSTITUENTS; CEREALS; ENZYMES; FOOD; GRASS; IMMUNOASSAY; KETO ACIDS; NUCLEIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; PEPTIDES; PHOSPHORUS-GROUP TRANSFERASES; PLANTS; PROTEINS; RNA; SYNTHESIS; TRANSFERASES; VEGETABLES; 550200* - Biochemistry

Citation Formats

Aoyagi, K, and Bassham, J A. Synthesis and uptake of cytoplasmically synthesized pyruvate, Pi dikinase polypeptide by chloroplasts. United States: N. p., 1985. Web. doi:10.1104/pp.78.4.807.
Aoyagi, K, & Bassham, J A. Synthesis and uptake of cytoplasmically synthesized pyruvate, Pi dikinase polypeptide by chloroplasts. United States. https://doi.org/10.1104/pp.78.4.807
Aoyagi, K, and Bassham, J A. 1985. "Synthesis and uptake of cytoplasmically synthesized pyruvate, Pi dikinase polypeptide by chloroplasts". United States. https://doi.org/10.1104/pp.78.4.807.
@article{osti_6026264,
title = {Synthesis and uptake of cytoplasmically synthesized pyruvate, Pi dikinase polypeptide by chloroplasts},
author = {Aoyagi, K and Bassham, J A},
abstractNote = {Polyadenylated RNA was isolated from maize leaves and translated in vitro. In agreement with a previous report by others, the authors found among the translation products a 110-kilodalton pyruvate orthophosphate dikinase (PPDK) precursor that is about 16 kilodaltons larger than the polypeptide isolated from cells. This maize PPDK precursor polypeptide was taken up from the translation product mixture by intact spinach chloroplasts and yielded a mature PPDK polypeptide (94 kilodaltons). The uptake and processing support the proposal that the extra 16-kilodalton size of the polypeptide from in vitro translation of maize leaf mRNA represents a transit sequence which is cleaved after its entry into chloroplasts. Moreover, these results provide additional evidence that in vivo in maize leaf cells PPDK polypeptide is synthesized in the cytoplasm and is transported into the chloroplasts. Location of PPDK in C/sub 3/ plant leaves was investigated by immunochemical analysis. A protein blot of stromal protein in each case gave rise to bands corresponding to authentic PPDK polypeptide. This result indicates that PPDK is present in chloroplasts of C/sub 3/ plant leaves as it is in the case of C/sub 4/ plants. 35 references, 3 figures.},
doi = {10.1104/pp.78.4.807},
url = {https://www.osti.gov/biblio/6026264}, journal = {Plant Physiol.; (United States)},
number = ,
volume = 78:4,
place = {United States},
year = {Thu Aug 01 00:00:00 EDT 1985},
month = {Thu Aug 01 00:00:00 EDT 1985}
}