Internode length in Pisum. Gene na may block gibberellin synthesis between ent-7. cap alpha. -hydroxykaurenoic acid and biggerellin A/sub 12/-aldehyde. [Pisum sativum]
The elongation response of the gibberellin (GA) deficient genotypes na, ls, and lh of peas (Pisum sativum L.) to a range of GA-precursors was examined. Plants possessing gene na did not respond to precursors in the GA biosynthetic pathway prior to GA/sub 12/-aldehyde. In contrast, plants possessing lh and ls responded as well as wild-type plants (dwarfed with AMO-1618) to these compounds. The results suggest that GA biosynthesis is blocked prior to ent-kaurene in the lh and ls mutants and between ent-7..cap alpha..-hydroxykaurenoic acid and GA/sub 12/-aldehyde in the na mutant. Feeds of ent(/sup 3/H)kaurenoic acid and (/sup 2/H)GA/sub 12/-aldehyde to a range of genotypes supported the above conclusions. The na line WL1766 was shown by gas chromatography-mass spectrometry (GC-MS) to metabolize(/sup 2/H)GA/sub 12/-aldehyde to a number of (/sup 2/H)C/sub 19/-GAs including GA/sub 1/. However, there was no indication in na genotypes for the metabolism of ent-(/sup 3/H)kaurenoic acid to these GAs. In contrast, the expanding shoot tissue of all Na genotypes examined metabolized ent-(/sup 3/H)kaurenoic acid to radioactive compounds that co-chromatographed with GA/sub 1/, GA/sub 8/, GA/sub 20/, and GA/sub 29/. However, insufficient material was present for unequivocal identification of the metabolites. The radioactive profiles from HPLC of extracts of the node treated with ent-(/sup 3/H)kaurenoic acid were similar for both Na and na plants and contained ent-16..cap alpha..,17-dihydroxykaurenoic acid and ent-6..cap alpha..,7..cap alpha..,16..beta..,17-tetrahydroxykaurenoic acid (both characterized by GC-MS), suggesting that the metabolites arose from side branches of the main GA-biosynthetic pathway. Thus, both Na and na plants appear capable of ent-7..cap alpha..-hydroxylation.
- Research Organization:
- Univ. of Bristol, England
- OSTI ID:
- 6020641
- Journal Information:
- Plant Physiol.; (United States), Vol. 83:4
- Country of Publication:
- United States
- Language:
- English
Similar Records
Thermoinductive regulation of gibberellin metabolism in Thlaspi arvense L
Comparison of sup 14 C-GA sub 12 - aldehyde metabolism in thermo- and non-induced shoot tips of Thlaspi arvense L
Related Subjects
GIBBERELLIC ACID
BIOSYNTHESIS
PLANT GROWTH
GENETIC CONTROL
BIOLOGICAL PATHWAYS
CHROMATOGRAPHY
DEUTERIUM COMPOUNDS
MASS SPECTROSCOPY
PHYSIOLOGY
PISUM
TRACER TECHNIQUES
TRITIUM COMPOUNDS
BACTERIA
CARBOXYLIC ACIDS
CONTROL
ESTERS
GROWTH
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LACTONES
LEGUMINOSAE
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEST CONTROL
PLANTS
RHIZOBIUM
SEPARATION PROCESSES
SPECTROSCOPY
SYNTHESIS
551001* - Physiological Systems- Tracer Techniques