Comparison of Th1- and Th2-associated immune reactivities stimulated by single versus multiple vaccination of mice with irradiated Schistosoma mansoni cercariae

Journal Article · Fri Mar 01 00:00:00 EST 1991 · Journal of Immunology; (USA)

OSTI ID:6007200

Caulada-Benedetti, Z; Al-Zamel, F; Sher, A; James, S ^[1]

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1. NIAID, Bethesda, MD (USA)

Mice immunized against Schistosoma mansoni by a single percutaneous exposure to radiation-attenuated parasite larvae demonstrate partial resistance to challenge infection that has been shown to correlate with development of cell-mediated immunity, whereas mice hyperimmunized by multiple exposure to attenuated larvae produce antibodies capable of transferring partial protection to naive recipients. Measurement of Ag-specific lymphokine responses in these animals suggested that the difference in resistance mechanisms may be due to the differential induction of Th subset response by the two immunization protocols. Thus, upon Ag stimulation, singly immunized mice predominantly demonstrated responses associated with Th1 reactivity, including IL-2 and IFN-gamma production, whereas multiply immunized animals showed increased IL-5, IL-4, and IgG1 antibody production associated with enhanced Th2 response. These responses demonstrated some degree of organ compartmentalization, with splenocytes demonstrating higher Th1-related lymphokine production and cells from draining lymph nodes showing stronger proliferation and Th2 type reactivity. However, hyperimmunized mice also continued to demonstrate substantial Th1-associated immune reactivity. Moreover, in vivo Ag challenge elicited activated larvacidal macrophages in hyperimmunized animals. These observations indicate that protective cell-mediated mechanisms associated with induction of CD4+ Th1 cell reactivity predominate in singly vaccinated mice. Further vaccination stimulates Th2 responses, such as enhanced IgG1 production, that may also contribute to protective immunity.

• OSTI ID: 6007200
• Journal Information: Journal of Immunology; (USA), Vol. 146:5; ISSN 0022-1767
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
LYMPHOCYTES
CELL PROLIFERATION
SCHISTOSOMIASIS
VACCINES
ANTIBODIES
IMMUNITY
INTERFERON
IRRADIATION
LARVAE
LYMPHOKINES
MACROPHAGES
MICE
SCHISTOSOMA
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
GROWTH FACTORS
HELMINTHS
INFECTIOUS DISEASES
LEUKOCYTES
MAMMALS
MATERIALS
MITOGENS
ORGANIC COMPOUNDS
PARASITIC DISEASES
PHAGOCYTES
PLATYHELMINTHS
PROTEINS
RODENTS
SOMATIC CELLS
TREMATODES
VERTEBRATES
553003* - Agriculture & Food Technology- Pest & Disease Control- (1987-)