Ozone toxicity effects on normal cultured lung cells and combined effects of ozone and antitumor drugs on normal and tumor cells
Abstract
A system was developed for investigating the effects of ozone (O/sub 3/) on cultured cells. Numerous factors were evaluated in designing this in vitro system and several factors, including composition of the liquid phase, depth of liquid covering cells and gas-liquid reaction products, were found to contribute significantly to the toxicity of O/sub 3/ for cultured rat lung fibroblasts (F-cells). Cell injury caused by different in vitro O/sub 3/ exposure methods was shown to be mediated by different toxic species. Cell injury, was mediated primarily by H/sub 2/O/sub 2/ with some contribution by O/sub 2//sup -/ and OH - depending upon the exposure method. Serum was found to protect from many of these O/sub 3/ reaction products by mechanisms involving protein-bound and free sulfhydryls. Combined treatment with O/sub 3/ and antitumor drugs was investigated for potential selective toxicity for tumor cells. Of the drugs tested, only vitamin K/sub 3/ (Vit K) produced a synergistic increase in toxicity for Walker rat carcinoma (WRC) cells when administered prior to or during O/sub 3/ exposure.
- Authors:
- Publication Date:
- OSTI Identifier:
- 5994661
- Resource Type:
- Thesis/Dissertation
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; OZONE; BIOLOGICAL EFFECTS; AIR POLLUTION; CELL CULTURES; COMPARATIVE EVALUATIONS; LUNGS; RATS; RESPONSE MODIFYING FACTORS; TOXICITY; VITAMIN K; ANIMALS; AROMATICS; BODY; COAGULANTS; DRUGS; HEMATOLOGIC AGENTS; HEPARIN ANTAGONISTS; MAMMALS; ORGANIC COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; ORGANS; POLLUTION; QUINONES; RESPIRATORY SYSTEM; RODENTS; VERTEBRATES; VITAMINS; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
Citation Formats
Morgan, D L.W. Ozone toxicity effects on normal cultured lung cells and combined effects of ozone and antitumor drugs on normal and tumor cells. United States: N. p., 1983.
Web.
Morgan, D L.W. Ozone toxicity effects on normal cultured lung cells and combined effects of ozone and antitumor drugs on normal and tumor cells. United States.
Morgan, D L.W. 1983.
"Ozone toxicity effects on normal cultured lung cells and combined effects of ozone and antitumor drugs on normal and tumor cells". United States.
@article{osti_5994661,
title = {Ozone toxicity effects on normal cultured lung cells and combined effects of ozone and antitumor drugs on normal and tumor cells},
author = {Morgan, D L.W.},
abstractNote = {A system was developed for investigating the effects of ozone (O/sub 3/) on cultured cells. Numerous factors were evaluated in designing this in vitro system and several factors, including composition of the liquid phase, depth of liquid covering cells and gas-liquid reaction products, were found to contribute significantly to the toxicity of O/sub 3/ for cultured rat lung fibroblasts (F-cells). Cell injury caused by different in vitro O/sub 3/ exposure methods was shown to be mediated by different toxic species. Cell injury, was mediated primarily by H/sub 2/O/sub 2/ with some contribution by O/sub 2//sup -/ and OH - depending upon the exposure method. Serum was found to protect from many of these O/sub 3/ reaction products by mechanisms involving protein-bound and free sulfhydryls. Combined treatment with O/sub 3/ and antitumor drugs was investigated for potential selective toxicity for tumor cells. Of the drugs tested, only vitamin K/sub 3/ (Vit K) produced a synergistic increase in toxicity for Walker rat carcinoma (WRC) cells when administered prior to or during O/sub 3/ exposure.},
doi = {},
url = {https://www.osti.gov/biblio/5994661},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 1983},
month = {Sat Jan 01 00:00:00 EST 1983}
}