Phosphorylation and associated translocation of the 87-kDa protein, a major protein kinase C substrate, in isolated nerve terminals
A protein of 87 kilodaltons (87 kDa) was previously identified as a major specific substrate for protein kinase C in neuronal and other tissues. We have now studied the effect of protein kinase C-catalyzed phosphorylation of this protein on its association with membranes in isolated nerve terminals (synaptosomes) from rat cerebral cortex. Incubation of synaptosomal membranes under conditions associated with activation of protein kinase C led to the release of the phosphorylated 87-kDa protein into the incubation medium. In intact synaptosomes, activation of protein kinase C by phorbol esters or by depolarization-induced Ca2+ influx caused an increased phosphorylation of the 87-kDa protein and its translocation from membrane to cytosol. This translocation showed time courses, calcium dependency, and reversibility similar to those observed for the protein kinase C-induced phosphorylation of the protein. These results suggest that protein kinase C-catalyzed phosphorylation of the 87-kDa protein is responsible for its subcellular translocation into the cytosol of nerve terminals.
- Research Organization:
- Rockefeller Univ., New York, NY (USA)
- OSTI ID:
- 5985790
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 86:7
- Country of Publication:
- United States
- Language:
- English
Similar Records
Ganglioside inhibition of glutamate-mediated protein kinase C translocation in primary cultures of cerebellar neurons
Role of protein kinase C (PKC) in short- and long-term cellular responses: inhibition of agonist-mediated calcium transients and down-regulation of PKC
Related Subjects
PHORBOL ESTERS
BIOLOGICAL EFFECTS
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
PROTEINS
PHOSPHORYLATION
CELL MEMBRANES
CEREBRAL CORTEX
MOLECULAR WEIGHT
NERVE CELLS
RATS
SUBSTRATES
ANIMAL CELLS
ANIMALS
BODY
BRAIN
CARCINOGENS
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CEREBRUM
CHEMICAL REACTIONS
ENZYMES
ESTERS
MAMMALS
MEMBRANES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS-GROUP TRANSFERASES
RODENTS
SOMATIC CELLS
TRANSFERASES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology