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Title: Restriction of cell surface expression of Sendai virus hemagglutinin-neuraminidase glycoprotein correlates with its higher instability in persistently and standard plus defective interfering virus infected BHK-21 cells

Abstract

To gain an understanding of the mechanism(s) by which Sendai virus generates a persistent infection, the expression of the hemagglutinin-neuraminidase (HN) and fusion (Fo) glycoproteins at the surfaces of BHK-21 cells infected with standard virus, a mixture of standard and defective interfering (DI) particles (mixed virus infection), and during persistent infection was investigated. The expression of HN and Fo was measured on the surfaces of infected cells by the binding of anti-HN and anti-Fo monoclonal antibodies. The results show that HN expression was restricted relative to Fo during mixed virus and persistent infections. The decreased levels of HN were investigated further by pulse-chase experiments which revealed that HN has an increased turnover rate in persistently infected cells and, to a lesser extent, in mixed virus infected cells. In analyzing the (/sup 35/S)methionine-labeled protein composition of virus particles produced during the pulse-chase experiments, the increased turnover of newly synthesized HN was found to correlate with its decreased incorporation into virus particles. Interestingly, the poor HN incorporation also correlates with less efficient incorporation of the matrix M protein into virus particles.

Authors:
; ;
Publication Date:
Research Org.:
Univ. of Geneva Medical School, Switzerland
OSTI Identifier:
5982982
Resource Type:
Journal Article
Journal Name:
Virology; (United States)
Additional Journal Information:
Journal Volume: 138:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HYDROLASES; ENZYME ACTIVITY; MONOCLONAL ANTIBODIES; CHEMICAL BONDS; VIRUSES; BIOCHEMISTRY; INFECTIVITY; ANTIGENS; GLUCOPROTEINS; HEMAGGLUTININS; LABELLED COMPOUNDS; METHIONINE; SULFUR 35; TRACER TECHNIQUES; AGGLUTININS; AMINO ACIDS; ANTIBODIES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOHYDRATES; CARBOXYLIC ACIDS; CHEMISTRY; DAYS LIVING RADIOISOTOPES; DRUGS; ENZYMES; EVEN-ODD NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; LIPOTROPIC FACTORS; MICROORGANISMS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PARASITES; PROTEINS; RADIOISOTOPES; SACCHARIDES; SULFUR ISOTOPES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Roux, L, Beffy, P, and Portner, A. Restriction of cell surface expression of Sendai virus hemagglutinin-neuraminidase glycoprotein correlates with its higher instability in persistently and standard plus defective interfering virus infected BHK-21 cells. United States: N. p., 1984. Web. doi:10.1016/0042-6822(84)90152-1.
Roux, L, Beffy, P, & Portner, A. Restriction of cell surface expression of Sendai virus hemagglutinin-neuraminidase glycoprotein correlates with its higher instability in persistently and standard plus defective interfering virus infected BHK-21 cells. United States. https://doi.org/10.1016/0042-6822(84)90152-1
Roux, L, Beffy, P, and Portner, A. 1984. "Restriction of cell surface expression of Sendai virus hemagglutinin-neuraminidase glycoprotein correlates with its higher instability in persistently and standard plus defective interfering virus infected BHK-21 cells". United States. https://doi.org/10.1016/0042-6822(84)90152-1.
@article{osti_5982982,
title = {Restriction of cell surface expression of Sendai virus hemagglutinin-neuraminidase glycoprotein correlates with its higher instability in persistently and standard plus defective interfering virus infected BHK-21 cells},
author = {Roux, L and Beffy, P and Portner, A},
abstractNote = {To gain an understanding of the mechanism(s) by which Sendai virus generates a persistent infection, the expression of the hemagglutinin-neuraminidase (HN) and fusion (Fo) glycoproteins at the surfaces of BHK-21 cells infected with standard virus, a mixture of standard and defective interfering (DI) particles (mixed virus infection), and during persistent infection was investigated. The expression of HN and Fo was measured on the surfaces of infected cells by the binding of anti-HN and anti-Fo monoclonal antibodies. The results show that HN expression was restricted relative to Fo during mixed virus and persistent infections. The decreased levels of HN were investigated further by pulse-chase experiments which revealed that HN has an increased turnover rate in persistently infected cells and, to a lesser extent, in mixed virus infected cells. In analyzing the (/sup 35/S)methionine-labeled protein composition of virus particles produced during the pulse-chase experiments, the increased turnover of newly synthesized HN was found to correlate with its decreased incorporation into virus particles. Interestingly, the poor HN incorporation also correlates with less efficient incorporation of the matrix M protein into virus particles.},
doi = {10.1016/0042-6822(84)90152-1},
url = {https://www.osti.gov/biblio/5982982}, journal = {Virology; (United States)},
number = ,
volume = 138:1,
place = {United States},
year = {Mon Oct 15 00:00:00 EDT 1984},
month = {Mon Oct 15 00:00:00 EDT 1984}
}