Specific proteins synthesized during the viral lytic cycle in vaccinia virus-infected HeLa cells: analysis by high-resolution, two-dimensional gel electrophoresis

Journal Article · Thu May 01 00:00:00 EDT 1986 · J. Virol.; (United States)

OSTI ID:5982678

Carrasco, L; Bravo, R

The proteins synthesized in vaccinia-infected HeLa cells have been analyzed at different times after infection by using two-dimensional gel electrophoresis. Vaccinia-infected cells present up to 198 polypeptides (138 acidic, isoelectric focusing; 60 basic, nonequilibrium pH gradient electrophoresis) not detected in control cells. Cells infected in the presence of cycloheximide show 81 additional polypeptides after cycloheximide removal, resulting in a total estimate of 279 proteins induced after vaccinia infection. The glycoproteins made at various time postinfection were also analyzed. At least 13 proteins labeled with (/sup 3/H)glucosamine were detected in vaccinia-infected HeLa cells.

• Research Organization: Universidad Autonoma, Madrid (Spain)
• OSTI ID: 5982678
• Journal Information: J. Virol.; (United States), Vol. 58:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PROTEINS
TWO-DIMENSIONAL ELECTROPHORESIS
VACCINIA VIRUS
BIOCHEMISTRY
BIOSYNTHESIS
CYCLOHEXIMIDE
DNA
GLUCOPROTEINS
GLUCOSAMINE
HELA CELLS
METHIONINE
SULFUR 35
TRITIUM COMPOUNDS
AMINES
AMINO ACIDS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
FUNGICIDES
GLYCOPROTEINS
HEXOSAMINES
HEXOSES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
MICROORGANISMS
MONOSACCHARIDES
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PESTICIDES
RADIOISOTOPES
SACCHARIDES
SULFUR ISOTOPES
SYNTHESIS
VIRUSES
550201* - Biochemistry- Tracer Techniques