Conversion of lignocellulosics pretreated with liquid hot water to ethanol
- Dartmouth College, Hanover, NH (United States); and others
Lignocellulosic materials pretreated using liquid hot water (LHW) (220{degrees}C, 5 MPa, 120 s) were fermented to ethanol by batch simultaneous saccharification and fermentation (SSF) using Saccharomyces cerevisiae in the presence of Trichoderma reesei cellulose. SSF of sugarcane bagasse (as received), aspen chips (smallest dimension 3 mm), and mixed hardwood flour (-60 +70 mesh) resulted in 90% conversion to ethanol in 2-5 d at enzyme loadings of 15-30 FPU/g. In most cases, 90% of the final conversion was achieved within 75 h of inoculation. Comminution of the pretreated substrates did not affect the conversion to ethanol. The hydrolysate produced from the LHW pretreatment showed slight inhibition of batch growth of S. cerevisiae. Solids pretreated at a concentration of 100 g/L were as reactive as those pretreated at a lower concentration, provided that the temperature was maintained at 220{degrees}C. 51 refs., 3 figs., 4 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 598205
- Report Number(s):
- CONF-950587-; ISSN 0273-2289; TRN: 98:001503-0003
- Journal Information:
- Applied Biochemistry and Biotechnology, Vol. 57-58; Conference: 17. symposium on biotechnology for fuels and chemicals, Vail, CO (United States), 7-11 May 1995; Other Information: PBD: Spr 1996
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
55 BIOLOGY AND MEDICINE
BASIC STUDIES
BAGASSE
CELLULOSE
COMMINUTION
BIOCONVERSION
ETHANOL FUELS
FERMENTATION
HOT WATER
INHIBITION
INOCULATION
SACCHARIFICATION
SACCHAROMYCES CEREVISIAE
SUBSTRATES
TRICHODERMA
RENEWABLE ENERGY SOURCES
BIOMASS
BATCH CULTURE
ENZYMATIC HYDROLYSIS
COST
EFFICIENCY
TEMPERATURE DEPENDENCE
CONCENTRATION RATIO
PARTICLE SIZE
REDUCTION