Reverse sample genome probing, a new technique for identification of bacteria in environmental samples by DNA hybridization, and its application to the identification of sulfate-reducing bacteria in oil field samples

Journal Article · Fri Nov 01 00:00:00 EST 1991 · Applied and Environmental Microbiology; (United States)

OSTI ID:5966980

Voordouw, G; Voordouw, J K; Karkhoff-Schweizer, R R ^[1]; Fedorak, P M; Westlake, D W.S. ^[2]

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1. Univ. of Calgary, Alberta (Canada)
2. Univ. of Alberta, Edmonton (Canada)

A novel method for identification of bacteria in environmental samples by DNA hybridization is presented. It is based on the fact that, even within a genus, the genomes of different bacteria may have little overall sequence homology. This allows the use of the labeled genomic DNA of a given bacterium (referred to as a standard) to probe for its presence and that of bacteria with highly homologous genomes in total DNA obtained from an environmental sample. Alternatively, total DNA extracted from the sample can be labeled and used to probe filters on which denatured chromosomal DNA from relevant bacterial standards has been spotted. The latter technique is referred to as reverse sample genome probing, since it is the reverse of the usual practice of deriving probes from reference bacteria for analyzing a DNA sample. Reverse sample genome probing allows identification of bacteria in a sample in a single step once a master filter with suitable standards has been developed. Application of reverse sample genome probing to the identification of sulfate-reducing bacteria in 31 samples obtained primarily from oil fields in the province of Alberta has indicated that there are at least 20 genotypically different sulfate-reducing bacteria in these samples.

• OSTI ID: 5966980
• Journal Information: Applied and Environmental Microbiology; (United States), Vol. 57:11; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

02 PETROLEUM
59 BASIC BIOLOGICAL SCIENCES
DNA
BIOASSAY
SULFATE-REDUCING BACTERIA
DNA HYBRIDIZATION
CLASSIFICATION
CORROSION
GENES
METALS
OIL FIELDS
OXIDOREDUCTASES
BACTERIA
CHEMICAL REACTIONS
ELEMENTS
ENZYMES
GEOLOGIC DEPOSITS
HYBRIDIZATION
MICROORGANISMS
MINERAL RESOURCES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PETROLEUM DEPOSITS
PROTEINS
RESOURCES
020900* - Petroleum- Environmental Aspects
550200 - Biochemistry