Bivariate flow cytometric analysis of murine intestinal epithelial cells for cytokinetic studies

Journal Article · Sun Jan 01 00:00:00 EST 1984 · Cytometry (Baltimore); (United States)

OSTI ID:5965803

Pallavicini, M G; Ng, C R; Gray, J W

The heterogeneous nature of the small intestine and the lack of methods to obtain pure crypt populations has, in the past, limited the application of standard flow cytometric analysis for cytokinetic studies of the proliferating crypts. The authors describe a flow cytometric technique to discriminate crypt and villus cells in an epithelial cell suspension on the basis of cell length, and to measure the DNA content of the discriminated subpopulations. These data indicate that bivariate analysis of a mixed epithelial cell suspension can be used to distinguish mature villus cells, G/sub 1/ crypt cells, and S-phase crypt cells. In addition, continuous labeling studies suggest that the position of a cell on the cell length axis reflects epithelial cell maturity. The authors applied this flow cytometric technique to determine the cytokinetic nature of epithelial cells obtained by sequential digestion of the small intestine. 22 references, 4 figures, 2 tables.

• Research Organization: Lawrence Livermore National Lab., CA
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 5965803
• Journal Information: Cytometry (Baltimore); (United States), Vol. 5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
EPITHELIUM
CELL PROLIFERATION
CELL FLOW SYSTEMS
DNA
EXPERIMENTAL DATA
MICE
MULTIVARIATE ANALYSIS
SMALL INTESTINE
ANIMAL TISSUES
ANIMALS
BODY
DATA
DIGESTIVE SYSTEM
GASTROINTESTINAL TRACT
INFORMATION
INTESTINES
MAMMALS
MATHEMATICS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
RODENTS
STATISTICS
TISSUES
VERTEBRATES
550300* - Cytology