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Title: Transforming growth factor (TGF)-. alpha. in human milk

Abstract

Transforming growth factor (TGF)-{alpha} and epidermal growth factor (EGF) were measured in human milk by means of homologous radioimmunoassay. As previously reported, EGF concentration in the colostrum was approximately 200 ng/ml and decreased to 50 ng/ml by day 7 postpartum. The value of immunoreactive (IR)-TGF-{alpha} was 2.2-7.2 ng/ml, much lower than that of EGF. In contrast to EGF, the concentration of IR-TGF-{alpha} was fairly stable during the 7 postpartum days. There was no relationship between the concentrations of IR-TGF-{alpha} and IR-EGF, suggesting that the regulatory mechanism in the release of the two growth factors is different. On gel-chromatography using a Sephadex G-50 column, IR-EGF appeared in the fraction corresponding to that of authentic human EGF, while 70%-80% of the IR-TGF-{alpha} was eluted as a species with a molecular weight greater than that of authentic human TGF-{alpha}. Although the physiological role of TGF-{alpha} in milk is not known, it is possible that it is involved in the development of the mammary gland and/or the growth of newborn infants.

Authors:
; ;  [1];  [2]; ; ; ; ;
  1. Research Institute for Growth Sciences, Tokyo (Japan)
  2. Tokyo Women's Medical College (Japan)
Publication Date:
OSTI Identifier:
5962990
Resource Type:
Journal Article
Journal Name:
Life Sciences; (USA)
Additional Journal Information:
Journal Volume: 48:12; Journal ID: ISSN 0024-3205
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GROWTH FACTORS; RADIOIMMUNOASSAY; BIOLOGICAL FUNCTIONS; BIOSYNTHESIS; GEL PERMEATION CHROMATOGRAPHY; MAN; MILK; MOLECULAR WEIGHT; ANIMALS; BIOASSAY; BIOLOGICAL MATERIALS; BODY FLUIDS; CHROMATOGRAPHY; DIAGNOSTIC TECHNIQUES; FOOD; FUNCTIONS; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; MAMMALS; MATERIALS; MITOGENS; ORGANIC COMPOUNDS; PRIMATES; PROTEINS; RADIOASSAY; RADIOIMMUNODETECTION; RADIOIMMUNOLOGY; SEPARATION PROCESSES; SYNTHESIS; TRACER TECHNIQUES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Okada, Masaki, Wakai, Kae, Shizume, Kazuo, Iwashita, Mitsutoshi, Ohmura, Eiji, Kamiya, Yoshinobu, Murakami, Hitomi, Onoda, Noritaka, and Tsushima, Toshio. Transforming growth factor (TGF)-. alpha. in human milk. United States: N. p., 1991. Web. doi:10.1016/0024-3205(91)90452-H.
Okada, Masaki, Wakai, Kae, Shizume, Kazuo, Iwashita, Mitsutoshi, Ohmura, Eiji, Kamiya, Yoshinobu, Murakami, Hitomi, Onoda, Noritaka, & Tsushima, Toshio. Transforming growth factor (TGF)-. alpha. in human milk. United States. https://doi.org/10.1016/0024-3205(91)90452-H
Okada, Masaki, Wakai, Kae, Shizume, Kazuo, Iwashita, Mitsutoshi, Ohmura, Eiji, Kamiya, Yoshinobu, Murakami, Hitomi, Onoda, Noritaka, and Tsushima, Toshio. 1991. "Transforming growth factor (TGF)-. alpha. in human milk". United States. https://doi.org/10.1016/0024-3205(91)90452-H.
@article{osti_5962990,
title = {Transforming growth factor (TGF)-. alpha. in human milk},
author = {Okada, Masaki and Wakai, Kae and Shizume, Kazuo and Iwashita, Mitsutoshi and Ohmura, Eiji and Kamiya, Yoshinobu and Murakami, Hitomi and Onoda, Noritaka and Tsushima, Toshio},
abstractNote = {Transforming growth factor (TGF)-{alpha} and epidermal growth factor (EGF) were measured in human milk by means of homologous radioimmunoassay. As previously reported, EGF concentration in the colostrum was approximately 200 ng/ml and decreased to 50 ng/ml by day 7 postpartum. The value of immunoreactive (IR)-TGF-{alpha} was 2.2-7.2 ng/ml, much lower than that of EGF. In contrast to EGF, the concentration of IR-TGF-{alpha} was fairly stable during the 7 postpartum days. There was no relationship between the concentrations of IR-TGF-{alpha} and IR-EGF, suggesting that the regulatory mechanism in the release of the two growth factors is different. On gel-chromatography using a Sephadex G-50 column, IR-EGF appeared in the fraction corresponding to that of authentic human EGF, while 70%-80% of the IR-TGF-{alpha} was eluted as a species with a molecular weight greater than that of authentic human TGF-{alpha}. Although the physiological role of TGF-{alpha} in milk is not known, it is possible that it is involved in the development of the mammary gland and/or the growth of newborn infants.},
doi = {10.1016/0024-3205(91)90452-H},
url = {https://www.osti.gov/biblio/5962990}, journal = {Life Sciences; (USA)},
issn = {0024-3205},
number = ,
volume = 48:12,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 1991},
month = {Tue Jan 01 00:00:00 EST 1991}
}