Detection of a unique antigen on radiation leukemia virus-induced leukemia B6RV2
Radiation leukemia virus-induced leukemia of a male C57BL/6 mouse, B6RV2, is immunogenic to female BALB/c X C57BL/6 F1 mice. In these mice, B6RV2 tumors regressed after initial growth, and after tumor regression the mice were resistant to repeated inocula of up to 10(8) B6RV2 cells. Serum from these mice reacted with B6RV2 in mixed hemadsorption or protein A assays, and absorption analysis indicated that the antigen was restricted to B6RV2; it could not be detected in normal thymocytes or spleen concanavalin A blasts from different inbred strains, nor in 16 C57BL/6 or BALB/c leukemias. Spleen cells from mice in which the tumor had regressed were cytotoxic to B6RV2 after in vitro stimulation with B6RV2, as shown by /sup 51/chromium release assay. This cytotoxicity was eliminated by pretreatment of the cells with anti-Thy-1.2, anti-Lyt-2.2, anti-Lyt-3.2, and complement, indicating that the effector cells were T-cells. The specificity of T-cell killing of B6RV2 was examined by competitive inhibition assays with unlabeled cells; only B6RV2 inhibited killing, while eight other C57BL/6 leukemias did not inhibit. Thus, the antigen on B6RV2 defined serologically and by cytotoxic T-cells is a unique antigen. However, it was not revealed by antibody-blocking test whether the unique determinant defined serologically was related to that recognized by T-cells; B6RV2 antiserum did not block lytic activity in the absence of added complement, irrespective of whether the target cells were untreated or anti-H-2b-treated B6RV2. H-2Kb antisera, but not H-2Db antisera, blocked lysis. This indicated that the H-2Kb molecule was exclusively involved in recognition of B6RV2 by cytotoxic T-cell.
- Research Organization:
- Center for Adult Diseases, Osaka, Japan
- OSTI ID:
- 5948705
- Journal Information:
- Cancer Res.; (United States), Vol. 44:11
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LEUKEMIA VIRUSES
ANTIGENS
MICE
ANTIGEN-ANTIBODY REACTIONS
CELL KILLING
CHROMIUM 51
IMMUNE REACTIONS
LEUKEMIA
NEOPLASMS
RADIOINDUCTION
SPLEEN CELLS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
CHROMIUM ISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HEMIC DISEASES
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
MICROORGANISMS
NUCLEI
ONCOGENIC VIRUSES
PARASITES
RADIOISOTOPES
RODENTS
SOMATIC CELLS
VERTEBRATES
VIRUSES
550901* - Pathology- Tracer Techniques
560152 - Radiation Effects on Animals- Animals