skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: (Summer investigations into the isolation, cultivation and metabolism of anaerobes involved in biodegradation): Progress report, year 4, summer 1988

Abstract

In the laboratory our students were trained in modern techniques for the isolation and study of a wide variety of microbes from marine and brackish environments. Special emphasis was placed on anaerobes and archaebacteria. Microbial groups that were studied included the propionic bacteria, clostridia, methanogens, acetogens, hydrogen oxidizing anaerobes and aerobes, sulfate-reducing bacteria and sulfur-reducing bacteria, anoxic photosynthetic bacteria, cyanobacteria, spirochetes, symbiotic and non-symbiotic nitrogen fixing bacteria, luminescent bacteria, iron bacteria, magnetic bacteria, and sulfur oxidizing bacteria. The permanent staff led discussions and presented lectures on the metabolism, physiology and biochemistry of the groups listed above. Material was also presented on motility and chemotaxis of bacteria, and particular emphasis was given to molecular approaches to studying evolution of bacteria. We also had five successful Microbiology Mini-symposia (see attached schedule). These one-day symposia involved lecture/seminar presentations by investigators involved in state-of-the-art working particularly exciting areas within the scope of our course.

Publication Date:
Research Org.:
Marine Biological Lab., Woods Hole, MA (USA)
OSTI Identifier:
5940402
Report Number(s):
DOE/ER/13361-4
ON: DE89015101
DOE Contract Number:
FG02-85ER13361
Resource Type:
Technical Report
Resource Relation:
Other Information: Portions of this document are illegible in microfiche products
Country of Publication:
United States
Language:
English
Subject:
58 GEOSCIENCES; FRESH WATER; ANAEROBIC CONDITIONS; SEAWATER; BACTERIA; MEETINGS; MICROORGANISMS; PROGRESS REPORT; TRAINING; DOCUMENT TYPES; HYDROGEN COMPOUNDS; OXYGEN COMPOUNDS; WATER; 580500* - Oceanography- (1980-1989)

Citation Formats

Not Available. (Summer investigations into the isolation, cultivation and metabolism of anaerobes involved in biodegradation): Progress report, year 4, summer 1988. United States: N. p., 1988. Web.
Not Available. (Summer investigations into the isolation, cultivation and metabolism of anaerobes involved in biodegradation): Progress report, year 4, summer 1988. United States.
Not Available. 1988. "(Summer investigations into the isolation, cultivation and metabolism of anaerobes involved in biodegradation): Progress report, year 4, summer 1988". United States. doi:.
@article{osti_5940402,
title = {(Summer investigations into the isolation, cultivation and metabolism of anaerobes involved in biodegradation): Progress report, year 4, summer 1988},
author = {Not Available},
abstractNote = {In the laboratory our students were trained in modern techniques for the isolation and study of a wide variety of microbes from marine and brackish environments. Special emphasis was placed on anaerobes and archaebacteria. Microbial groups that were studied included the propionic bacteria, clostridia, methanogens, acetogens, hydrogen oxidizing anaerobes and aerobes, sulfate-reducing bacteria and sulfur-reducing bacteria, anoxic photosynthetic bacteria, cyanobacteria, spirochetes, symbiotic and non-symbiotic nitrogen fixing bacteria, luminescent bacteria, iron bacteria, magnetic bacteria, and sulfur oxidizing bacteria. The permanent staff led discussions and presented lectures on the metabolism, physiology and biochemistry of the groups listed above. Material was also presented on motility and chemotaxis of bacteria, and particular emphasis was given to molecular approaches to studying evolution of bacteria. We also had five successful Microbiology Mini-symposia (see attached schedule). These one-day symposia involved lecture/seminar presentations by investigators involved in state-of-the-art working particularly exciting areas within the scope of our course.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 1988,
month = 1
}

Technical Report:
Other availability
Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that may hold this item. Keep in mind that many technical reports are not cataloged in WorldCat.

Save / Share:
  • Objectives of this project dealt with understanding one- and multicarbon metabolism in acetogenic and methanogenic bacteria. The biochemistry of acetate catabolism in Methanosarcina barkeri in relation to their major enzyme activities, co-factors and intermediates were studied. The physiological function of CO-dehydrogenase was investigated and found to be involved in hydrogen production when CO was used as substrate. Hydrogen metabolism from acetate by Methanosarcina barkeri was investigated through isotope tracer studies. Results showed that hydrogen production appears to be linked to several intracellular redox processes which follow the cleavage of acetate. The metabolic pathway in Butyribacterium methylotrophicum for multicarbon metabolism wasmore » elucidated. The key enzyme, ferredoxin-NAD reductase, was found to be regulated by NADH. The one carbon and multicarbon metabolic pathways in Butyribacterium methylotrophicum were constituted. Hydrogen was produced through multicarbon metabolism and was consumed by one carbon metabolism through the reduction of CO/sub 2/. Results revealed why high efficiency of carbon and energy conservation was observed in acetogens. The catabolism of formate to acetate by this microorganism was analyzed by fermentation time course and /sup 13/C nuclear magnetic resonance studies. Results indicate a bifurcated single-carbon catabolic pathway in which CO/sub 2/ is the sole single-carbon compound that directly supplies the carbonyl and methyl group synthesis pathways leading to the formation of acetyl coenzyme A, the primary reduced product. 1 fig.« less
  • This is the fourth annual preoperational Site Environmental Report for the Waste Isolation Pilot Plant (WIPP) in southeastern New Mexico. The purpose of the WIPP is to provide a research and development facility to demonstrate the safe disposal of transuranic (TRU) wastes generated by the defense activities of the US government. This document is prepared in accordance with the guidance which requires DOE facilities to ''submit an Annual Site Environmental Report to the Office of Operational Safety.''The report provides a comprehensive description of environmental activities at the WIPP during calendar year 1988, including a description of the WTPP project andmore » its mission; a description of the local environment, including demographics; a summary of environmental program information, including an update of the status of environmental permits and compliance activities; a presentation of the data of the Radiological Baseline Program (RBP), which is a program to characterize background radionuclide concentrations in the environment round the WIPP site; and a summary of findings of the Ecological Monitoring Program (EMP), which examines nonradiological impacts of WIPP construction on the surrounding ecosystem. 48 refs., 41 figs., 32 tabs.« less
  • Sclerotinia sclerotiorum contains D-erythroascorbic acid (EAA) and a closely related reducing acid, possibly the open-chain form of EAA. The organism cleaves one of these products or possibly both to yield OA and D-glyceric acid. The OA is rapidly secreted into the medium. An analogy can be made between AA-linked OA biosynthesis in higher plants and EAA-linked OA biosynthesis in fungi as exemplified by S. sclerotiorum.
  • Extremely thermophilic bacteria grow optimally between 80/degree/ and 105/degree/C. They were discovered only recently, little is known of their physiology and biochemistry, and no enzyme has been purified from them. However, they have enormous potential in energy conversions and the production of fuels and chemicals. In this project we are investigating the metabolism of molecular hydrogen (H/sub 2/) by five extremely thermophilic bacteria, selected on the basis of their remarkable physiology and remote phylogenetic positions. Two have been isolated from deep sea hypothermal vents. Our objectives are to grow these organisms on a large scale in vitro and purify theirmore » hydrogenases, the enzyme responsible for catalyzing H/sub 2/ activation and H/sub 2/ production, and related redox proteins. Their molecular and catalytic properties will then be determine by biochemical and spectroscopic techniques. The long term goal is to assess the biotechnological potential of these enzymes. 2 refs.« less
  • This booklet was assembled by the Committee on Training of Nuclear and Radiochemists of the Division of Nuclear Chemistry and Technology of the American Chemical Society as an aid to students interested in graduate studies in nuclear chemistry, radiochemistry, and related disciplines. The program descriptions contained herein were solicited from, and provided by, members of the Division. For additional information about graduate work, students are encouraged to contact the person designated at the bottom of each one-page description. The Directory of Graduate Research, published by the American Chemical Society, may also prove helpful in selecting an institution for graduate study.