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Title: Pulmonary fibrosis in experimental actue respiratory disease. [Rats]

Abstract

Changes in collagen metabolism were examined in 3 models of acute respiratory disease in rats. Fibrotic changes in the lungs of rats were provoked by exposing them to paraquat (intraperitoneal), ozone (inhaled), or bleomycin (intratracheally injected). After an interval sufficient to allow histologically discernible fibrosis to occur (6 to 7 days), lungs were removed from the rats, and apparent collagen synthesis rates were determined with cultured lung minces incubated in medium containing 3H-proline. Portions of the 3H-proline-labeled lung minces were then used for quantifying ratios of Type I to Type III collagen. There was no change in Type I/Type III collagen for total unlabeled collagen, nor was there any detectable increase of total collagen per lung after 1 week. We conclude that an early event in experimental acute respiratory disease is a marked increase in the relative synthesis of Type I collagen; this shift occurs before there is observable increased accumulation of collagen in the lung.

Authors:
;
Publication Date:
OSTI Identifier:
5940342
Resource Type:
Journal Article
Journal Name:
Am. Rev. Respir. Dis.; (United States)
Additional Journal Information:
Journal Volume: 123:1
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; BLEOMYCIN; BIOLOGICAL EFFECTS; COLLAGEN; BIOSYNTHESIS; CHROMATOGRAPHY; ELECTROPHORESIS; INSECTICIDES; OZONE; BIOLOGICAL MODELS; FIBROSIS; LUNGS; RATS; ANIMALS; ANTI-INFECTIVE AGENTS; ANTIBIOTICS; ANTIMITOTIC DRUGS; ANTINEOPLASTIC DRUGS; BODY; DRUGS; MAMMALS; ORGANIC COMPOUNDS; ORGANS; PATHOLOGICAL CHANGES; PESTICIDES; PROTEINS; RESPIRATORY SYSTEM; RODENTS; SCLEROPROTEINS; SEPARATION PROCESSES; SYNTHESIS; VERTEBRATES; 560306* - Chemicals Metabolism & Toxicology- Man- (-1987); 550200 - Biochemistry

Citation Formats

Reiser, K M, and Last, J A. Pulmonary fibrosis in experimental actue respiratory disease. [Rats]. United States: N. p., 1981. Web.
Reiser, K M, & Last, J A. Pulmonary fibrosis in experimental actue respiratory disease. [Rats]. United States.
Reiser, K M, and Last, J A. 1981. "Pulmonary fibrosis in experimental actue respiratory disease. [Rats]". United States.
@article{osti_5940342,
title = {Pulmonary fibrosis in experimental actue respiratory disease. [Rats]},
author = {Reiser, K M and Last, J A},
abstractNote = {Changes in collagen metabolism were examined in 3 models of acute respiratory disease in rats. Fibrotic changes in the lungs of rats were provoked by exposing them to paraquat (intraperitoneal), ozone (inhaled), or bleomycin (intratracheally injected). After an interval sufficient to allow histologically discernible fibrosis to occur (6 to 7 days), lungs were removed from the rats, and apparent collagen synthesis rates were determined with cultured lung minces incubated in medium containing 3H-proline. Portions of the 3H-proline-labeled lung minces were then used for quantifying ratios of Type I to Type III collagen. There was no change in Type I/Type III collagen for total unlabeled collagen, nor was there any detectable increase of total collagen per lung after 1 week. We conclude that an early event in experimental acute respiratory disease is a marked increase in the relative synthesis of Type I collagen; this shift occurs before there is observable increased accumulation of collagen in the lung.},
doi = {},
url = {https://www.osti.gov/biblio/5940342}, journal = {Am. Rev. Respir. Dis.; (United States)},
number = ,
volume = 123:1,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1981},
month = {Thu Jan 01 00:00:00 EST 1981}
}