Assessment of preferential cleavage of an actively transcribed retroviral hybrid gene in murine cells by deoxyribonuclease I, bleomycin, neocarzinostatin, or ionizing radiation
Preferential cleavage induced by bleomycin, neocarzinostatin, or ionizing radiation in a transcribed cellular gene was evaluated through comparisons with deoxyribonuclease I. The glucocorticoid-inducible LTL gene previously described served as the specific DNA target. A Southern blot analysis was used to specifically assess cleavage of the LTL gene in nuclei isolated from cells either treated or untreated with the synthetic glucocorticoid dexamethasone. Hypersensitivity of the gene to bleomycin or neocarzinostatin, which paralleled deoxyribonuclease I hypersensitivity, was evident only in nuclei isolated from dexamethasone-treated cells. Like deoxyribonuclease I, sites of dexamethasone-inducible drug hypersensitivity were coincident with the binding region for the glucocorticoid receptor found within the regulatory sequences of the LTL gene. In contrast, no hypersensitivity to ionizing radiation was evident. Although bleomycin and neocarzinostatin showed qualitatively similar preferences for the threshold LTL gene, quantitative evaluations of damage to total cellular DNA by filter elution showed that the relative specificity of bleomycin for the hypersensitive region was much less than that of either deoxyribonuclease I or neocarzinostatin.
- Research Organization:
- Roswell Park Memorial Institute, Buffalo, NY
- OSTI ID:
- 5934727
- Journal Information:
- Biochemistry; (United States), Vol. 26:17
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BLEOMYCIN
BIOLOGICAL EFFECTS
DNA
BIOLOGICAL RADIATION EFFECTS
ENZYMATIC HYDROLYSIS
NEOCARCINOSTATIN
CARBON 14 COMPOUNDS
DNA-ASE
GENES
PHOSPHORUS 32
VIRUSES
X RADIATION
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
HYDROLASES
HYDROLYSIS
IONIZING RADIATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LYSIS
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
PHOSPHORUS ISOTOPES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOMIMETIC DRUGS
SOLVOLYSIS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
560300 - Chemicals Metabolism & Toxicology