Structural peculiarities of rat liver DNA during inhibition of protein synthesis by cycloheximide

Journal Article · Mon Jan 20 00:00:00 EST 1986 · Biochemistry (Engl. Transl.); (United States)

OSTI ID:5933835

Fonarev, A B; Shugalii, A V; Papina, R I; Todorov, I N

Curves of the reassociation of rat liver DNA, isolated 12-48 h after the animals were given a sublethal dose of CHI, were obtained in the region of the parameter c/sub 0/t up to 20. For summary preparations of DNA-24 and DNA-36 at c/sub 0/t 0.02-0.6, an increase in the contribution of the rapidly reassociating sequences by an average of 4% was recorded. For individual kinetic fractions the differences in the rate of reassociation reach the 2.5-3-fold level. The total incorporation of the label of the DNA-24 preparation is sharply reduced; however, there is a substantial heterogeneity of the incorporation for fractions differentiated according to the parameter c/sub 0/t.

• Research Organization: Institute of Chemical Physics, Chernogolovka, USSR
• OSTI ID: 5933835
• Journal Information: Biochemistry (Engl. Transl.); (United States), Vol. 50:7; Other Information: Translation from Biokhimiya; 50: No. 7, 1162-1166(Jul 1985)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CYCLOHEXIMIDE
BIOLOGICAL EFFECTS
PROTEINS
BIOSYNTHESIS
RECOMBINANT DNA
AMINO ACID SEQUENCE
GENE RECOMBINATION
LABELLING
TRACER TECHNIQUES
BIOCHEMICAL REACTION KINETICS
DOSE-RESPONSE RELATIONSHIPS
INHIBITION
LIVER
RATS
THYMIDINE
TRITIUM COMPOUNDS
ULTRASTRUCTURAL CHANGES
UPTAKE
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
AZINES
BODY
DIGESTIVE SYSTEM
DNA
DRUGS
FUNGICIDES
GLANDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MOLECULAR STRUCTURE
MORPHOLOGICAL CHANGES
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PESTICIDES
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
RODENTS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques