Role of glutamine in cobinamide biosynthesis in Propionibacterium shermanii

Journal Article · Sat Jan 10 00:00:00 EST 1987 · Biochemistry (Engl. Transl.); (United States)

OSTI ID:5933657

Eliseev, A A; Pushkin, A V; Belozerova, E V; Bykhovskii, V Ya

The role of glutamine as a possible donor of amide groups in the biosynthesis of vitamin B/sub 12/ was investigated. In the incubation of P. shermanii cells preliminarily exhausted with respect to nitrogen on media containing ammonium sulfate or asparagine, the glutamine synthetase inhibitor methionine sulfoximine suppressed the formation of cobinamide (factor B) from the monoamide of cobiric acid (by 75 and 59%, respectively). At the same time, the inhibitor did not affect cobinamide synthesis on a medium with glutamine. The amide group of glutamine, labeled with /sup 13/N, was used for the amidation of corrinoids four times as efficiently as the amine group. It was concluded that a glutamine-dependent synthetase, which catalyzes the amidation of cobiric acids with the formation of cobinamide, functions in cells of propionic acid bacteria.

• Research Organization: A.N. Bakh Institute of Biochemistry, Moscow, USSR
• OSTI ID: 5933657
• Journal Information: Biochemistry (Engl. Transl.); (United States), Vol. 51:7; Other Information: Translated from Biokhimiya; 51: No. 7, 1072-1076(Jul 1986)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUTAMINE
BIOCHEMISTRY
BIOCONVERSION
RADIOENZYMATIC ASSAY
LIGASES
ENZYME ACTIVITY
VITAMIN B-12
BIOSYNTHESIS
BACTERIA
BINDING ENERGY
CELL CULTURES
INCUBATION
LABELLED COMPOUNDS
LABELLING
NITROGEN 15
SUBSTRATES
AMIDES
AMINO ACIDS
CARBOXYLIC ACIDS
CHEMISTRY
DRUGS
ENERGY
ENZYMES
HEMATINICS
HEMATOLOGIC AGENTS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NITROGEN ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
STABLE ISOTOPES
SYNTHESIS
VITAMIN B GROUP
VITAMINS
550301* - Cytology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques