The response of a human bronchial epithelial cell line to histamine: Intracellular calcium changes and extracellular release of inflammatory mediators
- Department of Pediatrics, University of North Carolina School of Medicine, Chapel Hill (United States)
Epithelial cells are likely to modulate inflammation and tissue repair in the airways, but the factors responsible for these processes remain unclear. Because human airway epithelia are infrequently available for in vitro studies, transformed epithelial cell lines are of interest as models. The authors therefore investigated the response of an SV-40/adenovirus-transformed human bronchial epithelial cell line (BEAS-2B) to histamine, a mediator with relevance for airway diseases. The intracellular calcium response to histamine (10(-4) M) was measured, using Fura-2 and microspectrofluorimetry. Histamine induced a transient increase in intracellular calcium that originated from intracellular sources; this effect was inhibited by the H1 receptor antagonist diphenhydramine, suggesting that BEAS cells retain functioning histamine receptors. BEAS cells were grown to confluence on microporous, collagen-coated filters, allowing measurement of vectorial release of soluble mediators. Monolayers exposed to histamine for 30 min released interleukin-6 and fibronectin in the apical direction, in a dose-dependent manner. Little eicosanoid production was induced by histamine, either in the apical or the basolateral direction, although BEAS cells constitutively produced small amounts of prostaglandin E2 and 15-HETE. However, these cells formed large amounts of eicosanoids in response to ozone exposure as a positive control. Comparison of their data with published reports for human airway epithelia in primary culture suggests that the BEAS cell line is, in a number of respects, a relevant model for the study of airway epithelial responses to a variety of stimuli.
- OSTI ID:
- 5929363
- Journal Information:
- American Journal of Respiratory Cell and Molecular Biology; (United States), Vol. 5:5; ISSN 1044-1549
- Country of Publication:
- United States
- Language:
- English
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ANIMAL CELLS
SENSITIVITY
HISTAMINE
BIOLOGICAL EFFECTS
OZONE
BRONCHI
BRONCHITIS
CALCIUM
DOSE-RESPONSE RELATIONSHIPS
EPITHELIUM
MAN
RECEPTORS
ALKALINE EARTH METALS
AMINES
ANIMAL TISSUES
ANIMALS
AZOLES
BODY
DISEASES
ELEMENTS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
MAMMALS
MEMBRANE PROTEINS
METALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PROTEINS
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
TISSUES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology