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Title: The separation of a mixture of bone marrow stem cells from tumor cells: an essential step for autologous bone marrow transplantation

Abstract

KHT tumor cells were mixed with mouse bone marrow to simulate a sample of bone marrow containing metastatic tumor cells. This mixture was separated into a bone marrow fraction and a tumor cell fraction by centrifugal elutriation. Elutriation did not change the transplantability of the bone marrow stem cells as measured by a spleen colony assay and an in vitro erythroid burst forming unit assay. The tumorogenicity of the KHT cells was similarly unaffected by elutriation. The data showed that bone marrow cells could be purified to less than 1 tumor cell in more than 10/sup 6/ bone marrow cells. Therefore, purification of bone marrow removed prior to lethal radiation-drug combined therapy for subsequent autologous transplantation appears to be feasible using modifications of this method if similar physical differences between human metastatic tumor cells and human bone marrow cells exist. This possibility is presently being explored.

Authors:
 [1]; ; ; ;
  1. Univ. of Rochester, NY
Publication Date:
OSTI Identifier:
5923555
Resource Type:
Journal Article
Journal Name:
Int. J. Radiat. Oncol., Biol. Phys.; (United States)
Additional Journal Information:
Journal Volume: 7:10
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; BONE MARROW; TRANSPLANTS; BONE MARROW CELLS; ELUTRIATION; TUMOR CELLS; CENTRIFUGATION; INERTIAL SEPARATORS; MICE; WHOLE-BODY IRRADIATION; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; BODY; CONNECTIVE TISSUE CELLS; EQUIPMENT; EXTERNAL IRRADIATION; HEMATOPOIETIC SYSTEM; IRRADIATION; MAMMALS; ORGANS; POLLUTION CONTROL EQUIPMENT; RODENTS; SEPARATION EQUIPMENT; SEPARATION PROCESSES; SOMATIC CELLS; TISSUES; VERTEBRATES; 550603* - Medicine- External Radiation in Therapy- (1980-)

Citation Formats

Rubin, P, Wheeler, K T, Keng, P C, Gregory, P K, and Croizat, H. The separation of a mixture of bone marrow stem cells from tumor cells: an essential step for autologous bone marrow transplantation. United States: N. p., 1981. Web. doi:10.1016/0360-3016(81)90037-7.
Rubin, P, Wheeler, K T, Keng, P C, Gregory, P K, & Croizat, H. The separation of a mixture of bone marrow stem cells from tumor cells: an essential step for autologous bone marrow transplantation. United States. https://doi.org/10.1016/0360-3016(81)90037-7
Rubin, P, Wheeler, K T, Keng, P C, Gregory, P K, and Croizat, H. 1981. "The separation of a mixture of bone marrow stem cells from tumor cells: an essential step for autologous bone marrow transplantation". United States. https://doi.org/10.1016/0360-3016(81)90037-7.
@article{osti_5923555,
title = {The separation of a mixture of bone marrow stem cells from tumor cells: an essential step for autologous bone marrow transplantation},
author = {Rubin, P and Wheeler, K T and Keng, P C and Gregory, P K and Croizat, H},
abstractNote = {KHT tumor cells were mixed with mouse bone marrow to simulate a sample of bone marrow containing metastatic tumor cells. This mixture was separated into a bone marrow fraction and a tumor cell fraction by centrifugal elutriation. Elutriation did not change the transplantability of the bone marrow stem cells as measured by a spleen colony assay and an in vitro erythroid burst forming unit assay. The tumorogenicity of the KHT cells was similarly unaffected by elutriation. The data showed that bone marrow cells could be purified to less than 1 tumor cell in more than 10/sup 6/ bone marrow cells. Therefore, purification of bone marrow removed prior to lethal radiation-drug combined therapy for subsequent autologous transplantation appears to be feasible using modifications of this method if similar physical differences between human metastatic tumor cells and human bone marrow cells exist. This possibility is presently being explored.},
doi = {10.1016/0360-3016(81)90037-7},
url = {https://www.osti.gov/biblio/5923555}, journal = {Int. J. Radiat. Oncol., Biol. Phys.; (United States)},
number = ,
volume = 7:10,
place = {United States},
year = {Thu Oct 01 00:00:00 EDT 1981},
month = {Thu Oct 01 00:00:00 EDT 1981}
}