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Title: Cessation of respiration after far-ultraviolet irradiation of Escherichia coli B/r: loss of unaltered pyridine nucleotides to the medium

Abstract

Cessation of respiration of Escherichia coli B/r cells is initiated 30 min after irradiation at 254 nm and is linked to cell death. Pyridine nucleotides begin to disappear with the onset of respiratory failure and are almost completely absent from the cells by 90 min after irradiation. We studied the fate of these respiratory cofactors in a niacin-requiring mutant (RSI) grown on minimal medium containing (7-/sup 14/C)nicotinic acid. By 90 min after irradiation (52 J/m/sup 2/) nearly all of the acid-soluble radioactive counts appeared in the medium. Paper chromatographic studies and a spectrophotometric assay indicated that the material was nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate. The loss of nicotinamide adenine dinucleotide was not balanced by synthesis, despite the presence of appropriate active biosynthetic enzymes for at least 90 min after uv irradiation. Analysis of the amino acid and nucleotide pool of the cells showed that there was some loss of most of these small molecules; the levels of a few were almost completely depleted. We conclude that the pyridine nucleotides are lost from the cell to the medium and that the loss cannot be attributed to extensive general membrane damage.

Authors:
 [1]; ;
  1. Oak Ridge National Lab., TN
Publication Date:
OSTI Identifier:
5917319
DOE Contract Number:  
W-7405-ENG-26
Resource Type:
Journal Article
Journal Name:
Radiat. Res.; (United States)
Additional Journal Information:
Journal Volume: 79:3
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ESCHERICHIA COLI; FAR ULTRAVIOLET RADIATION; BIOLOGICAL EFFECTS; NAD; NADP; BIOLOGICAL PATHWAYS; CARBON 14; CELL KILLING; INHIBITION; NUCLEOTIDES; PYRIDINE; RESPIRATION; AZINES; BACTERIA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBON ISOTOPES; COENZYMES; ELECTROMAGNETIC RADIATION; EVEN-EVEN NUCLEI; HETEROCYCLIC COMPOUNDS; ISOTOPES; LIGHT NUCLEI; MICROORGANISMS; NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIDINES; RADIATIONS; RADIOISOTOPES; ULTRAVIOLET RADIATION; YEARS LIVING RADIOISOTOPES; 550201* - Biochemistry- Tracer Techniques; 560400 - Other Environmental Pollutant Effects

Citation Formats

Schenley, R L, Swenson, P A, and Joshi, J G. Cessation of respiration after far-ultraviolet irradiation of Escherichia coli B/r: loss of unaltered pyridine nucleotides to the medium. United States: N. p., 1979. Web. doi:10.2307/3575185.
Schenley, R L, Swenson, P A, & Joshi, J G. Cessation of respiration after far-ultraviolet irradiation of Escherichia coli B/r: loss of unaltered pyridine nucleotides to the medium. United States. https://doi.org/10.2307/3575185
Schenley, R L, Swenson, P A, and Joshi, J G. Sat . "Cessation of respiration after far-ultraviolet irradiation of Escherichia coli B/r: loss of unaltered pyridine nucleotides to the medium". United States. https://doi.org/10.2307/3575185.
@article{osti_5917319,
title = {Cessation of respiration after far-ultraviolet irradiation of Escherichia coli B/r: loss of unaltered pyridine nucleotides to the medium},
author = {Schenley, R L and Swenson, P A and Joshi, J G},
abstractNote = {Cessation of respiration of Escherichia coli B/r cells is initiated 30 min after irradiation at 254 nm and is linked to cell death. Pyridine nucleotides begin to disappear with the onset of respiratory failure and are almost completely absent from the cells by 90 min after irradiation. We studied the fate of these respiratory cofactors in a niacin-requiring mutant (RSI) grown on minimal medium containing (7-/sup 14/C)nicotinic acid. By 90 min after irradiation (52 J/m/sup 2/) nearly all of the acid-soluble radioactive counts appeared in the medium. Paper chromatographic studies and a spectrophotometric assay indicated that the material was nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate. The loss of nicotinamide adenine dinucleotide was not balanced by synthesis, despite the presence of appropriate active biosynthetic enzymes for at least 90 min after uv irradiation. Analysis of the amino acid and nucleotide pool of the cells showed that there was some loss of most of these small molecules; the levels of a few were almost completely depleted. We conclude that the pyridine nucleotides are lost from the cell to the medium and that the loss cannot be attributed to extensive general membrane damage.},
doi = {10.2307/3575185},
url = {https://www.osti.gov/biblio/5917319}, journal = {Radiat. Res.; (United States)},
number = ,
volume = 79:3,
place = {United States},
year = {1979},
month = {9}
}