Calcium fluxes across the plasma membrane of Commelina communis L. assayed in a cell-free system
- Ruhr-Universitaet Bochum (West Germany)
The inside-out fraction of plasma membrane-rich vesicles prepared from leaves of Commelina communis L. by aqueous two-phase partitioning was loaded with {sup 45}Ca{sup 2+} through the action of the plasma membrane Ca{sup 2+}-ATPase. Results suggest the presence of a Ca{sup 2+} channel in the plasma membrane of C. communis. The channel is obtained in a Ca{sup 2+}-inactivated state after preparation and Ca{sup 2+}-loading of the vesicles. The inactivation is removed by TFP (trifluoperazine) or W-7 (N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide), presumably due to the Ca{sup 2+}-mobilizing effect of these compounds. The activated Ca{sup 2+} channel is La{sup 3+} sensitive and, in the cell, would allow for passage of Ca{sup 2+} into the cell. The possibility that TFP or W-7 act independent of CM, or through CM tightly associated with the plasma membrane, is discussed.
- OSTI ID:
- 5914772
- Journal Information:
- Plant Physiology; (USA), Vol. 93:3; ISSN 0032-0889
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CALCIUM COMPOUNDS
MEMBRANE TRANSPORT
HERBS
PHYSIOLOGY
ATP-ASE
CALCIUM 45
CATIONS
CELL MEMBRANES
ION CHANNELING
LANTHANUM COMPOUNDS
LEAVES
ACID ANHYDRASES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM ISOTOPES
CELL CONSTITUENTS
CHANNELING
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
HYDROLASES
INTERMEDIATE MASS NUCLEI
IONS
ISOTOPES
MEMBRANES
NUCLEI
PHOSPHOHYDROLASES
PLANTS
RADIOISOTOPES
RARE EARTH COMPOUNDS
551001* - Physiological Systems- Tracer Techniques