skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Technology assessment and strategy for development of a Rapid Field Water Microbiology Test Kit. Technical report

Abstract

A literature and market search of existing technology for the detection, identification, and quantification of microorganisms in water was conducted. Based upon the availability of technologies and their configurations, an assessment of the appropriate strategies to pursue for the near and long term development plans in development of the Rapid Field Bacteriology Test Kit was performed. Near term technologies to improve the Army's capability to detect microorganisms would appear to be essentially improvements in versatility and measurement of coliform indicator organisms. New chromogenic and fluorogenic indicator substances associated with new substrates appear to be best suited for test kit development either for quantitative membrane filter tests or presence/absence and multiple fermentation tests. Test times, incubator requirements, and operator involvement appear to be similar to older technologies. Long term development would appear to favor such technologies as genetic probes with amplification of the hydridized nucleic acid materials of positive samples, and some immunological based systems such as enzyme linked, immuno-sorbent assays. In both cases, the major problems would appear to be sample preparation and development of signal strengths from the reactions which would allow the user to see results in 1 hour.

Authors:
;
Publication Date:
Research Org.:
Army Biomedical Research and Development Lab., Fort Detrick, MD (United States)
OSTI Identifier:
5910792
Alternate Identifier(s):
OSTI ID: 5910792
Report Number(s):
AD-A-243413/2/XAB; USABRDL-TR--9106
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ESCHERICHIA COLI; SENSITIVITY; WATER POLLUTION; BIOLOGICAL INDICATORS; ENZYME IMMUNOASSAY; FIELD TESTS; NUCLEIC ACIDS; PLANNING; SAMPLING; SUBSTRATES; TECHNOLOGY ASSESSMENT; BACTERIA; BIOASSAY; IMMUNOASSAY; MICROORGANISMS; ORGANIC COMPOUNDS; POLLUTION; TESTING 540320* -- Environment, Aquatic-- Chemicals Monitoring & Transport-- (1990-); 560300 -- Chemicals Metabolism & Toxicology

Citation Formats

Preston, D.R., and Schaub, S.A. Technology assessment and strategy for development of a Rapid Field Water Microbiology Test Kit. Technical report. United States: N. p., 1991. Web.
Preston, D.R., & Schaub, S.A. Technology assessment and strategy for development of a Rapid Field Water Microbiology Test Kit. Technical report. United States.
Preston, D.R., and Schaub, S.A. Sun . "Technology assessment and strategy for development of a Rapid Field Water Microbiology Test Kit. Technical report". United States.
@article{osti_5910792,
title = {Technology assessment and strategy for development of a Rapid Field Water Microbiology Test Kit. Technical report},
author = {Preston, D.R. and Schaub, S.A.},
abstractNote = {A literature and market search of existing technology for the detection, identification, and quantification of microorganisms in water was conducted. Based upon the availability of technologies and their configurations, an assessment of the appropriate strategies to pursue for the near and long term development plans in development of the Rapid Field Bacteriology Test Kit was performed. Near term technologies to improve the Army's capability to detect microorganisms would appear to be essentially improvements in versatility and measurement of coliform indicator organisms. New chromogenic and fluorogenic indicator substances associated with new substrates appear to be best suited for test kit development either for quantitative membrane filter tests or presence/absence and multiple fermentation tests. Test times, incubator requirements, and operator involvement appear to be similar to older technologies. Long term development would appear to favor such technologies as genetic probes with amplification of the hydridized nucleic acid materials of positive samples, and some immunological based systems such as enzyme linked, immuno-sorbent assays. In both cases, the major problems would appear to be sample preparation and development of signal strengths from the reactions which would allow the user to see results in 1 hour.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1991},
month = {9}
}

Technical Report:
Other availability
Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that may hold this item. Keep in mind that many technical reports are not cataloged in WorldCat.

Save / Share: