Comparative effects of glyphosate and atrazine in chloroplast ultrastructure of wheat and downy brome. [Triticum aestivum; Bromus tectorum]
Developing and mature leaves of winter wheat (Triticum aestivum L. var. Daws) and the weed species downy brome (Bromus tectorum L.) were subjected to 10 mM (foliar application) and 1 mM (root application) herbicide solutions. Glyphosate (N-(phosphonomethyl) glycine) and atrazine (2-chloro-4-(ethyl-amino)-6-(isopropylamino)-s-triazine) were prepared in a carrier composed of 5% soybean oil concentrate, 35% acetone and 60% water. Penetration experiments with /sup 3/H-labelled herbicides assessed what percentage of herbicide entered leaves, and microautoradiography was used to determine qualitatively how much herbicide was present in the sections viewed with TEM. Tissue was excised at 4, 18, 62 and 200 hours, and then either freeze-substituted or fixed chemically. Ultrastructural effects of each herbicide on chloroplasts from leaves of newly-germinated seedlings and of well-tillered plants are depicted and discussed. Temporal differences in response of chloroplasts to each herbicide are noted.
- Research Organization:
- Dept. of Agriculture-ARS, Pullman, WA
- OSTI ID:
- 5897337
- Report Number(s):
- CONF-8707108-; TRN: 87-037639
- Journal Information:
- Plant Physiol., Suppl.; (United States), Vol. 83:4; Conference: Annual meeting of the American Society of Plant Physiologists, St. Louis, MO, USA, 19 Jul 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
60 APPLIED LIFE SCIENCES
HERBICIDES
ABSORPTION
BIOLOGICAL EFFECTS
AUTORADIOGRAPHY
CHLOROPLASTS
LEAVES
ROOT ABSORPTION
SEEDLINGS
TRACER TECHNIQUES
TRANSMISSION ELECTRON MICROSCOPY
TRITIUM COMPOUNDS
WEEDS
WHEAT
CELL CONSTITUENTS
CEREALS
ELECTRON MICROSCOPY
GRASS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROSCOPY
PESTICIDES
PLANTS
UPTAKE
560300* - Chemicals Metabolism & Toxicology
553006 - Agriculture & Food Technology- Other Agricultural Applications- (1987-)