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Title: Temperature programming in capillary zone electrophoresis

Abstract

A now type of selectivity in capillary zone electrophoresis (CZE) based on temperature programming is presented. By using a buffer system with a large temperature coefficient, e.g. Tris buffer, the pH within the capillary can be adjusted in situ by simply controlling the temperature of the capillary. The electromigration behavior of weak acids is found to be influenced by both temperature-induced pH changes and viscosity changes. The effect of pH on the electrophoretic mobilities of analytes is most significant if the range of pH change matches the acidity constants, pK{sub a}, of the analytes. The implementation of temperature programming in both temporal and positional modes in CZE are illustrated by the separation of a mixture of fluorescent organic acids. 29 refs., 4 figs., 2 tabs.

Authors:
;  [1]
  1. Iowa State Univ., Ames, IA (United States)
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
589058
DOE Contract Number:  
W-7405-ENG-82
Resource Type:
Journal Article
Journal Name:
Analytical Chemistry (Washington)
Additional Journal Information:
Journal Volume: 64; Journal Issue: 5; Other Information: PBD: 1 Mar 1992
Country of Publication:
United States
Language:
English
Subject:
40 CHEMISTRY; SEPARATION PROCESSES; ELECTROPHORESIS; ORGANIC ACIDS; TEMPERATURE COEFFICIENT; VISCOSITY

Citation Formats

Whang, Chen-Wen, and Yeung, E S. Temperature programming in capillary zone electrophoresis. United States: N. p., 1992. Web. doi:10.1021/ac00029a010.
Whang, Chen-Wen, & Yeung, E S. Temperature programming in capillary zone electrophoresis. United States. https://doi.org/10.1021/ac00029a010
Whang, Chen-Wen, and Yeung, E S. Sun . "Temperature programming in capillary zone electrophoresis". United States. https://doi.org/10.1021/ac00029a010.
@article{osti_589058,
title = {Temperature programming in capillary zone electrophoresis},
author = {Whang, Chen-Wen and Yeung, E S},
abstractNote = {A now type of selectivity in capillary zone electrophoresis (CZE) based on temperature programming is presented. By using a buffer system with a large temperature coefficient, e.g. Tris buffer, the pH within the capillary can be adjusted in situ by simply controlling the temperature of the capillary. The electromigration behavior of weak acids is found to be influenced by both temperature-induced pH changes and viscosity changes. The effect of pH on the electrophoretic mobilities of analytes is most significant if the range of pH change matches the acidity constants, pK{sub a}, of the analytes. The implementation of temperature programming in both temporal and positional modes in CZE are illustrated by the separation of a mixture of fluorescent organic acids. 29 refs., 4 figs., 2 tabs.},
doi = {10.1021/ac00029a010},
url = {https://www.osti.gov/biblio/589058}, journal = {Analytical Chemistry (Washington)},
number = 5,
volume = 64,
place = {United States},
year = {1992},
month = {3}
}