Temperature programming in capillary zone electrophoresis

doi:https://doi.org/10.1021/ac00029a010

Title: Temperature programming in capillary zone electrophoresis

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Abstract

A now type of selectivity in capillary zone electrophoresis (CZE) based on temperature programming is presented. By using a buffer system with a large temperature coefficient, e.g. Tris buffer, the pH within the capillary can be adjusted in situ by simply controlling the temperature of the capillary. The electromigration behavior of weak acids is found to be influenced by both temperature-induced pH changes and viscosity changes. The effect of pH on the electrophoretic mobilities of analytes is most significant if the range of pH change matches the acidity constants, pK{sub a}, of the analytes. The implementation of temperature programming in both temporal and positional modes in CZE are illustrated by the separation of a mixture of fluorescent organic acids. 29 refs., 4 figs., 2 tabs.

Authors:

Whang, Chen-Wen; Yeung, E S ^[1]

Iowa State Univ., Ames, IA (United States)

Publication Date:: 1992-03-01

Sponsoring Org.:: USDOE

OSTI Identifier:: 589058

DOE Contract Number:: W-7405-ENG-82

Resource Type:: Journal Article

Journal Name:: Analytical Chemistry (Washington)

Additional Journal Information:: Journal Volume: 64; Journal Issue: 5; Other Information: PBD: 1 Mar 1992

Country of Publication:: United States

Language:: English

Subject:: 40 CHEMISTRY; SEPARATION PROCESSES; ELECTROPHORESIS; ORGANIC ACIDS; TEMPERATURE COEFFICIENT; VISCOSITY

Citation Formats


                    Whang, Chen-Wen, and Yeung, E S. Temperature programming in capillary zone electrophoresis.  United States: N. p., 1992. 
        Web.  doi:10.1021/ac00029a010.

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                    Whang, Chen-Wen, & Yeung, E S. Temperature programming in capillary zone electrophoresis.  United States.  https://doi.org/10.1021/ac00029a010

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                    Whang, Chen-Wen, and Yeung, E S. Sun .  
        "Temperature programming in capillary zone electrophoresis".  United States.  https://doi.org/10.1021/ac00029a010.

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@article{osti_589058,

  title        = {Temperature programming in capillary zone electrophoresis},

  author       = {Whang, Chen-Wen and Yeung, E S},

  abstractNote = {A now type of selectivity in capillary zone electrophoresis (CZE) based on temperature programming is presented. By using a buffer system with a large temperature coefficient, e.g. Tris buffer, the pH within the capillary can be adjusted in situ by simply controlling the temperature of the capillary. The electromigration behavior of weak acids is found to be influenced by both temperature-induced pH changes and viscosity changes. The effect of pH on the electrophoretic mobilities of analytes is most significant if the range of pH change matches the acidity constants, pK{sub a}, of the analytes. The implementation of temperature programming in both temporal and positional modes in CZE are illustrated by the separation of a mixture of fluorescent organic acids. 29 refs., 4 figs., 2 tabs.},

  doi          = {10.1021/ac00029a010},

  url          = {https://www.osti.gov/biblio/589058},
  journal      = {Analytical Chemistry (Washington)},
number       = 5,

  volume       = 64,

  place        = {United States},

  year         = {1992},

  month        = {3}

}

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