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Title: Determination of cyanide using a microbial sensor

Abstract

A microbial cyanide sensor was prepared, consisting of immobilized Saccharomyces cerevisiae and an oxygen electrode. When the electrode was inserted into a solution containing glucose, the respiration activity of the microorganisms increased. The change in the respiration activity is monitored with the oxygen electrode. When cyanide is added to the sample solution, the electron transport chain reaction of the respiration system in the mitochondria is inhibited, resulting in a decrease in respiration. The inhibition is caused by cyanide binding with respiration enzymes such as the cytochrome oxidase complex in the mitochondrial inner membrane. Therefore, the cyanide concentration can be measured from the change in the respiration rate. When the sensor was applied to a batch system at pH 8.0 and 30{degrees}C, the cyanide calibration curve showed linearity in the concentration range between 0.3 pM and 150 {mu}m CN{sup -}. 13 refs., 8 figs., 1 tab.

Authors:
; ;  [1]
  1. Univ. of Tokyo (Japan)
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
588725
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied Biochemistry and Biotechnology; Journal Volume: 60; Journal Issue: 2; Other Information: PBD: Aug 1996
Country of Publication:
United States
Language:
English
Subject:
44 INSTRUMENTATION, INCLUDING NUCLEAR AND PARTICLE DETECTORS; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; CALIBRATION; CYTOCHROME OXIDASE; ENZYMES; GLUCOSE; MICROORGANISMS; RANGE; RESPIRATION; PH VALUE; TEMPERATURE DEPENDENCE; SACCHAROMYCES CEREVISIAE; CYANIDES; DETECTION; MONITORING; ELECTRODES; CONCENTRATION RATIO; CORRELATIONS; WASTE PROCESSING; TOXIC MATERIALS; SEPARATION PROCESSES

Citation Formats

Nakanishi, Keijiro, Ikebukuro, Kazunori, and Karube, Isao. Determination of cyanide using a microbial sensor. United States: N. p., 1996. Web. doi:10.1007/BF02788064.
Nakanishi, Keijiro, Ikebukuro, Kazunori, & Karube, Isao. Determination of cyanide using a microbial sensor. United States. doi:10.1007/BF02788064.
Nakanishi, Keijiro, Ikebukuro, Kazunori, and Karube, Isao. Thu . "Determination of cyanide using a microbial sensor". United States. doi:10.1007/BF02788064.
@article{osti_588725,
title = {Determination of cyanide using a microbial sensor},
author = {Nakanishi, Keijiro and Ikebukuro, Kazunori and Karube, Isao},
abstractNote = {A microbial cyanide sensor was prepared, consisting of immobilized Saccharomyces cerevisiae and an oxygen electrode. When the electrode was inserted into a solution containing glucose, the respiration activity of the microorganisms increased. The change in the respiration activity is monitored with the oxygen electrode. When cyanide is added to the sample solution, the electron transport chain reaction of the respiration system in the mitochondria is inhibited, resulting in a decrease in respiration. The inhibition is caused by cyanide binding with respiration enzymes such as the cytochrome oxidase complex in the mitochondrial inner membrane. Therefore, the cyanide concentration can be measured from the change in the respiration rate. When the sensor was applied to a batch system at pH 8.0 and 30{degrees}C, the cyanide calibration curve showed linearity in the concentration range between 0.3 pM and 150 {mu}m CN{sup -}. 13 refs., 8 figs., 1 tab.},
doi = {10.1007/BF02788064},
journal = {Applied Biochemistry and Biotechnology},
number = 2,
volume = 60,
place = {United States},
year = {Thu Aug 01 00:00:00 EDT 1996},
month = {Thu Aug 01 00:00:00 EDT 1996}
}
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