Cloning of a marine cyanobacterial promoter for foreign gene expression using a promoter probe vector
- Tokyo Univ. of Agriculture and Technology (Japan)
A marine cyanobacterial promoter was cloned to allow efficient foreign gene expression. This was carried out using chloramphenicol acetyl transferase (CAT) as a marker protein. For rapid and simple measurement of CAT activity, a method based on a fluorescently labeled substrate was improved by utilizing HPLC equipped with a flow-through fluorescent spectrophotometer. This method was used in conjunction with a newly constructed promoter probe vector. Cyanobacterial transformants, harboring plasmid containing a cloned 2-kbp marine cyanobacterial genomic fragment, showed a 10-fold higher CAT activity, compared with that achieved using the kanamycin-resistant gene promoter. From the sequence analysis of the cloned fragment, a putative promoter region was found. 20 refs., 7 figs., 2 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 588692
- Journal Information:
- Applied Biochemistry and Biotechnology, Vol. 59, Issue 3; Other Information: PBD: Jun 1996
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
08 HYDROGEN FUEL
54 ENVIRONMENTAL SCIENCES
CHLORAMPHENICOL
DNA-CLONING
GENES
GENE REGULATION
CYANOBACTERIA
GENE REPRESSORS
GENETIC ENGINEERING
PRODUCTION
HYDROGEN
NUCLEOTIDES
BIOLOGICAL MARKERS
PROTEINS
SPECTROPHOTOMETRY
FLUORESCENCE
PLASMIDS
DNA SEQUENCING
SOLAR ENERGY CONVERSION
CARBON DIOXIDE
CARBON DIOXIDE FIXATION