Electrospray ionization mass spectrometry and tandem mass spectrometry of small oligonucleotides

Conference · Mon May 01 00:00:00 EDT 1989

OSTI ID:5881392

Edmonds, C. G.; Barinaga, C. J.; Loo, J. A.; Udseth, H. R.; Smith, R. D.

We have carried out a preliminary evaluation of electrospray ionization (ESI) mass spectrometry for the analysis of small oligonucleotides. In addition to the qualitative and quantitative potential suggested by the success of ESI of peptides and proteins, ESI tandem mass spectrometry may also be amenable of large oligonucleotides is usually performed by chemical degradation or by the chain termination technique. Smaller oligonucleotides have been analyzed by mass spectrometry with advantages in speed and simplicity of analysis. Tandem spectrometry has been effectively employed for the singly charged molecular anions of oligonucleotides to a polymerization level of n = 6/sup 4/. Fenn and co-workers have reported the ESI of nucleotide mono-, di- and triphosphates. A ESI mass spectrum of a deoxynucleotide 14-mer has also been recently reported.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 5881392
• Report Number(s): PNL-SA-16632; CONF-8905166-7; ON: DE89014926
• Resource Relation: Conference: American Society for Mass Spectroscopy annual symposium, Miami Beach, FL, USA, 22-26 May 1989; Other Information: Portions of this document are illegible in microfiche products
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC
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MASS SPECTROMETERS
MOLECULAR IONS
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ORGANIC NITROGEN COMPOUNDS
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