High performance liquid chromatography in enzymatic analysis: Applications to the assay of enzymatic activity

Book · Thu Jan 01 00:00:00 EST 1987

OSTI ID:5880766

Rossomando, E F

The author describes the high performance liquid chromatography (hplc) method and explains and illustrates its use in the analysis of enzymatic activities, rather than its traditional use as a tool for separation. This change in emphasis is particularly attractive to those who wish to make use of the activity of an enzyme as an indicator of cellular function, a determinant of a given stage of differentiation (or dedifferentiation), or even as a measure of gene function. Inherent in the hplc technique is the advantage of analyzing an activity after only a minimal amount of purification. Presentation focuses on the assay of the activity of an enzyme rather than its purification. Each chapter deals with a different aspect of the method, beginning with an overview and ending with a detailed summary. The final chapter treats the growing field of assay of multienzyme systems. The book includes more than 170 illustrations reproduced form the original research literature.

• OSTI ID: 5880766
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
ENZYME ACTIVITY
ENZYMES
BIOASSAY
BIOLOGICAL FUNCTIONS
LIQUID COLUMN CHROMATOGRAPHY
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOLOGICAL PATHWAYS
CELLULOLYTIC ACTIVITY
EVALUATION
GENES
CHEMISTRY
CHROMATOGRAPHY
FUNCTIONS
KINETICS
REACTION KINETICS
SEPARATION PROCESSES
550200* - Biochemistry
550400 - Genetics
400105 - Separation Procedures